PXD047416 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mapping myddosome components in human and murine macrophages. |
Description | Toll-like Receptors (TLRs) are key mediators of immune responses to infection. Upon microbial detection, these receptors activate inflammatory signal transduction pathways that involve IκB kinases, mitogen activated protein kinases, ubiquitin ligases and other adaptor proteins. Current models suggest these signaling proteins operate within functionally distinct multiprotein complexes, which are all activated by a receptor-proximal complex known as the myddosome. The mechanisms that connect the effector protein complexes in the TLR pathways are undefined. To define TLR pathway activities, we engineered macrophages to enable proteomic analysis of the endogenous myddosome constituent MyD88 and its interacting proteins. Proteomics demonstrated that myddosomes contain proteins that act at all stages and regulate all effector responses of the TLR signaling pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-04 |
AnnouncementXML | Submission_2024-07-04_10:59:15.349.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniel Fisch |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-30 13:45:08 | ID requested | |
⏵ 1 | 2024-07-04 10:59:15 | announced | |
Publication List
Keyword List
submitter keyword: LPS, myddosome, APEX, TMT, human, MyD88, proximity-biotinylation, Toll-like receptor, mouse, PAMP,Macrophage, P3C |
Contact List
Jonathan C Kagan |
contact affiliation | Division of Gastroenterology, Boston Children's Hospital and Harvard Medical School, 300 Longwood Ave., Boston, MA 02115, USA |
contact email | jonathan.kagan@childrens.harvard.edu |
lab head | |
Daniel Fisch |
contact affiliation | Boston Children's Hospital |
contact email | daniel.fisch@crick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047416
- Label: PRIDE project
- Name: Mapping myddosome components in human and murine macrophages.