PXD047376-2

PXD047376 is an original dataset announced via ProteomeXchange.

Title	R-proteins in modification deficient ribosomes - free 50S subunits
Description	Ribosomal RNA modifications are introduced by specific enzymes during ribosome assembly in bacteria. Deletion of individual modification enzymes has a minor effect on bacterial growth, ribosome biogenesis, and translation, which has complicated the definition of the function of the enzymes and their products. We have constructed an E. coli strain lacking 10 genes encoding enzymes that modify 23S rRNA around the peptidyl-transferase center. This strain exhibits severely compromised growth and ribosome assembly, especially at lower temperatures. Ribosomal protein composition of the mature 50S ribosomes and free 50S subunits was analyzed by SILAC based qMS approach. We have found that absence of 23S rRNA modifications around PTC does not affect significantly r-protein content of incompletely assembled 50S or mature 50S ribosomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:33:32.017.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047376
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kaspar Reier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	(4,4,5,5-(2)H4)-L-lysine; monohydroxylated residue; 6x(13)C labeled L-arginine; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-29 05:07:00	ID requested
1	2024-10-07 13:51:27	announced
⏵ 2	2024-10-22 06:33:32	announced	2024-10-22: Updated project metadata.

10.1093/NAR/GKAE222;

10.6019/PXD047376;

submitter keyword: r-protein,E.coli ribosome

Tiina Tamm
contact affiliation	Chair of Molecular Biology Institute of Cellular and Molecular Biology Estonia
contact email	ttamm@ut.ee
lab head
Kaspar Reier
contact affiliation	University of Tartu
contact email	kaspar.reier@ut.ee
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047376
     1. Label: PRIDE project
     2. Name: R-proteins in modification deficient ribosomes - free 50S subunits