PXD047349-2

PXD047349 is an original dataset announced via ProteomeXchange.

Title	Discovery of novel proteins in Agrobacterium tumefaciens to improve molecular pharming yields of Trastuzumab
Description	PlantForm Inc. (https://www.plantformcorp.com) is a Canadian biopharmaceutical company that produces antibody drugs using molecular pharming, where a genetically modified strain of Agrobacterium tumefaciens (strain At564A, derived from C58) transforms a genetically modified strain of Nicotiana benthamiana KDFX plant line (derived from USDA accession “TW16”) for biopharmaceutical expression. However, further research is required to comprehensively profile the proteome and secretome of At564A in their pipeline to identify areas of improvement. Using mass spectrometry-based bottom-up proteomics, we identified significantly altered abundance of virulence proteins after inducing virulence using acetosyringone and plant leaf extract from Nicotiana benthamiana.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:32.354.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kiyan Kheradvar
SpeciesList	scientific name: Agrobacterium fabrum str. C58; NCBI TaxID: 176299;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-28 13:43:28	ID requested
1	2024-10-15 01:50:10	announced
⏵ 2	2024-10-22 07:01:32	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Cancer,Agrobacterium tumefaciens, Biopharmaceuticals, Proteomics, LC-MS/MS

Prof. Jennifer Geddes-McAlister
contact affiliation	Associate Professor, Molecular and Cellular Biology Department, University of Guelph
contact email	jgeddesm@uoguelph.ca
lab head
Kiyan Kheradvar
contact affiliation	University of Guelph
contact email	kiyankred@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD047349

PRIDE project URI

• PRIDE
  1. PXD047349
     1. Label: PRIDE project
     2. Name: Discovery of novel proteins in Agrobacterium tumefaciens to improve molecular pharming yields of Trastuzumab