PXD047323 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of human platelet-derived extracellular vesicles generated via activation by different receptors |
| Description | Renowned for their role in hemostasis and thrombosis, platelets are increasingly recognized for their involvement in innate immunity, immunothrombosis and inflammatory diseases. Platelets, heterogeneous in size and molecular content, express a wide range of receptors enabling versatile activation endpoints relevant for immune functions. Upon activation, platelet release extracellular vesicles (PEVs), whose formation and molecular cargo exhibit remarkable tunability based on receptor-mediated activation or environmental cues. This study aimed to compare the immune modulatory roles of PEVs released through activation by specific platelet receptors, GPVI, CLEC-2, and thrombin-collagen (TC). Functional assays in vivo in zebrafish and using an in vitro human macrophage models highlighted distinct immune-inflammatory responses triggered by PEVs. Surprisingly, despite extensive characterization using omics analyses and basic EV particle characterization, the differences in the protein and miRNA cargo and the EV physicochemical properties between the PEV types were subtle and insufficient to precisely define their mechanism of action to distinguish a distinct functional profile. Moreover, the constitutively formed PEVs used as controls displayed a disparate activation profile from the receptor-induced PEVs. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-07 |
| AnnouncementXML | Submission_2024-10-07_02:38:27.723.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mari Palviainen |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | acetylated residue; monohydroxylated residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-11-28 03:57:42 | ID requested | |
| 1 | 2024-09-19 04:12:38 | announced | |
| 2 | 2024-10-07 02:34:02 | announced | 2024-10-07: Updated project metadata. |
| ⏵ 3 | 2024-10-07 02:38:28 | announced | 2024-10-07: Updated project metadata. |
| 4 | 2024-10-22 06:59:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: extracellular vesicles,human, platelet |
Contact List
| Pia Siljander |
|---|
| contact affiliation | University of Helsinki, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, Helsinki, Finland |
| contact email | pia.siljander@helsinki.fi |
| lab head | |
| Mari Palviainen |
|---|
| contact affiliation | University of Helsinki |
| contact email | mari.palviainen@helsinki.fi |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD047323 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047323
- Label: PRIDE project
- Name: Proteomic analysis of human platelet-derived extracellular vesicles generated via activation by different receptors
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