PXD047291 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chronic stress alters the E/I balance of pyramidal neurons in the infralimbic and Prelimbic cortices |
Description | The medial prefrontal cortex (mPFC) is a key brain region involved in controlling working memory, executive function, and self-regulatory behaviours, and its malfunction is a hallmark of several neuropsychiatric disorders such as schizophrenia, depression, and post-traumatic stress disorder. One of the main risk factors for the development of mental illness is chronic stress (CS). Despite some evidence pointing to possible excitation-inhibition (E/I) imbalances caused by CS, the precise mechanism by which CS triggers mPFC dysfunction is not fully understood. Here, using neuroproteomics analysis and whole-cell patch-clamp recordings, we investigated the functional changes in E/I ratio and synaptic drive onto pyramidal neurons and parvalbumin interneurons (PV) in the prelimbic (PL) and infralimbic (IL) cortices, following exposure to 21 days of chronic unpredictable stress. We demonstrate that CS impacts PL- versus IL-pyramidal neurons differently, driving a common decrease in E/I ratio without affecting PV interneurons. Our work brings insightful information and corroborates the hypothesis of stress-induced hypofunction of the mPFC. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_02:49:53.249.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bruno Manadas |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidoethyl-L-cysteine |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-27 13:23:13 | ID requested | |
⏵ 1 | 2024-10-17 02:49:54 | announced | |
Publication List
10.1523/eneuro.0053-24.2024; |
Rodrigues D, Santa C, Manadas B, Monteiro P, Chronic Stress Alters Synaptic Inhibition/Excitation Balance of Pyramidal Neurons But Not PV Interneurons in the Infralimbic and Prelimbic Cortices of C57BL/6J Mice. eNeuro, 11(8):(2024) [pubmed] |
Keyword List
submitter keyword: Infralimbic Cortex,Chronic stress, Prelimbic Cortex, E/I balance |
Contact List
Patricia Monteiro |
contact affiliation | Department of Biomedicine, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal. |
contact email | pmonteiro@med.up.pt |
lab head | |
Bruno Manadas |
contact affiliation | Center for Neurosciences and Cell Biology, University of Coimbra |
contact email | bmanadas@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047291
- Label: PRIDE project
- Name: Chronic stress alters the E/I balance of pyramidal neurons in the infralimbic and Prelimbic cortices