PXD047291-1

PXD047291 is an original dataset announced via ProteomeXchange.

Title	Chronic stress alters the E/I balance of pyramidal neurons in the infralimbic and Prelimbic cortices
Description	The medial prefrontal cortex (mPFC) is a key brain region involved in controlling working memory, executive function, and self-regulatory behaviours, and its malfunction is a hallmark of several neuropsychiatric disorders such as schizophrenia, depression, and post-traumatic stress disorder. One of the main risk factors for the development of mental illness is chronic stress (CS). Despite some evidence pointing to possible excitation-inhibition (E/I) imbalances caused by CS, the precise mechanism by which CS triggers mPFC dysfunction is not fully understood. Here, using neuroproteomics analysis and whole-cell patch-clamp recordings, we investigated the functional changes in E/I ratio and synaptic drive onto pyramidal neurons and parvalbumin interneurons (PV) in the prelimbic (PL) and infralimbic (IL) cortices, following exposure to 21 days of chronic unpredictable stress. We demonstrate that CS impacts PL- versus IL-pyramidal neurons differently, driving a common decrease in E/I ratio without affecting PV interneurons. Our work brings insightful information and corroborates the hypothesis of stress-induced hypofunction of the mPFC.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:49:53.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bruno Manadas
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 13:23:13	ID requested
⏵ 1	2024-10-17 02:49:54	announced

10.1523/eneuro.0053-24.2024;

Rodrigues D, Santa C, Manadas B, Monteiro P, Chronic Stress Alters Synaptic Inhibition/Excitation Balance of Pyramidal Neurons But Not PV Interneurons in the Infralimbic and Prelimbic Cortices of C57BL/6J Mice. eNeuro, 11(8):(2024) [pubmed]

submitter keyword: Infralimbic Cortex,Chronic stress, Prelimbic Cortex, E/I balance

Patricia Monteiro
contact affiliation	Department of Biomedicine, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal.
contact email	pmonteiro@med.up.pt
lab head
Bruno Manadas
contact affiliation	Center for Neurosciences and Cell Biology, University of Coimbra
contact email	bmanadas@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047291
     1. Label: PRIDE project
     2. Name: Chronic stress alters the E/I balance of pyramidal neurons in the infralimbic and Prelimbic cortices