PXD047212-1

PXD047212 is an original dataset announced via ProteomeXchange.

Title	Advancing Understanding of the Protein Composition of Human Seminal Extracellular Vesicles
Description	Seminal extracellular vesicles (SEVs) carry a diverse array of bioactive molecules, including proteins, lipids, and nucleic acids, which influence sperm function and have potential to modulate the female reproductive tract immune response after intromission. However, the full spectrum of SEV cargo involved in these processes remains incompletely defined. Here, we employed label-free quantitative high-resolution mass spectrometry to characterize the human SEV proteome, identifying 5079 associated proteins. These proteins were shown to likely originate from multiple regions of the male reproductive tract, notably the seminal vesicles and prostate, providing evidence for heterogeneous tissue origins of SEVs. Bioinformatic analysis revealed enrichment in sperm- and immune-related functions, as well as functions linked to protein translation. Notably, we identified several proteins with established roles in sperm physiology and immune signaling that had not previously been linked with SEV function. These included; Adenylate kinase isoenzyme (AK)2/9, and Calcium-binding tyrosine-phosphorylation regulated protein (CABYR), implicated in sperm motility, and immune regulators such as the Toll-like receptor 4 ligand, High mobility group protein B1 (HMGB1), and the Nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) inhibitor epsilon (NFκBIE). Altogether, these findings expand the known SEV proteome and highlight proteins that may influence both male and female reproductive capacity.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_21:14:34.947.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047212
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-23 20:22:17	ID requested
⏵ 1	2025-12-14 21:14:36	announced

Tamessar CT, Parameswaran S, Torres-Arce E, Weidenhofer J, Zhang HM, Mulhall JE, Robertson SA, Bromfield EG, Nixon B, Sharkey DJ, Skerrett-Byrne DA, Schjenken JE, Advancing Understanding of the Protein Composition of Human Seminal Extracellular Vesicles. Proteomics, ():e70088(2025) [pubmed]

10.6019/PXD047212;

10.1002/pmic.70088;

submitter keyword: None

John Schjenken
contact affiliation	School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia. Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia.
contact email	John.Schjenken@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	Helmholtz Zentrum München
contact email	david.skerrett-byrne@newcastle.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047212
     1. Label: PRIDE project
     2. Name: Advancing Understanding of the Protein Composition of Human Seminal Extracellular Vesicles