PXD047199-1

PXD047199 is an original dataset announced via ProteomeXchange.

Title	Staphylococcus aureus mutants resistant to the feed-additive monensin show increased virulence and altered purine metabolism
Description	Ionophores are antibacterial compounds that affect bacterial growth by changing intracellular concentrations of the essential cations, sodium and potassium. They are extensively used in animal husbandry to increase productivity and reduce infectious diseases. Given their widespread usage, it is important to determine the potential negative consequences of ionophore use on human and animal health. In this study, we demonstrate that exposure to the ionophore monensin can select for resistant mutants in the human and animal pathogen Staphylococcus aureus, with a majority of the resistant mutants showing increased growth rates in vitro and/or in mice. Whole-genome sequencing and proteomics analysis of the resistant mutants show that the resistance phenotype is associated with de-repression of de novo purine synthesis, which could be achieved through mutations in different transcriptional regulators including mutations in the gene purR, the repressor of the purine de novo synthesis pathway. This study shows that mutants with reduced susceptibility to the ionophore monensin can be readily selected and highlights an unexplored link between ionophore resistance, purine metabolism and fitness in pathogenic bacteria.
HostingRepository	PRIDE
AnnounceDate	2024-01-09
AnnouncementXML	Submission_2024-01-09_05:01:36.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evelin Berger
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-23 06:36:11	ID requested
⏵ 1	2024-01-09 05:01:37	announced

Dataset with its publication pending

submitter keyword: virulence, Monensin, purine metabolism, S. aureus,Ionophore resistance

Carina Sihlbom Wallem
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	carina.sihlbom@gu.se
lab head
Evelin Berger
contact affiliation	University of Gothenburg
contact email	evelin.berger@gu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047199
     1. Label: PRIDE project
     2. Name: Staphylococcus aureus mutants resistant to the feed-additive monensin show increased virulence and altered purine metabolism