PXD047167-1

PXD047167 is an original dataset announced via ProteomeXchange.

Title	A single gene mutation underpins metabolic adaptation and acquisition of filamentous competence in the emerging fungal pathogen Candida auris
Description	Filamentous cell growth is a vital property of fungal pathogens. The mechanisms of filamentation in the emerging multidrug-resistant fungal pathogen Candida auris are poorly understood. Here, we show that exposure of C. auris to glycerol triggers a rod-like filamentation-competent (RL-FC) phenotype, which forms elongated filamentous cells after a prolonged culture period. Whole-genome sequencing analysis reveals that all RL-FC isolates harbor a mutation in the C2H2 zinc finger transcription factor-encoding gene GFC1 (Gfc1 variants). Deletion of GFC1 leads to an RL-FC phenotype similar to that observed in Gfc1 variants. We further demonstrate that GFC1 mutation causes enhanced fatty acid β-oxidation metabolism and thereby promotes RL-FC/filamentous growth. This regulation is achieved through a Multiple Carbon source Utilizer (Mcu1)-dependent mechanism. Interestingly, both the evolved RL-FC isolates and the gfc1Δ mutant exhibit an enhanced ability to colonize the skin. Our results reveal that glycerol-mediated GFC1 mutations are beneficial during C. auris skin colonization and infection.
HostingRepository	PRIDE
AnnounceDate	2024-06-12
AnnouncementXML	Submission_2024-06-12_05:47:22.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchen Deng
SpeciesList	scientific name: Fungi; NCBI TaxID: NCBITaxon:4751; scientific name: [Candida] auris; NCBI TaxID: 498019; scientific name: Candida; NCBI TaxID: NCBITaxon:5475;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-22 04:13:58	ID requested
⏵ 1	2024-06-12 05:47:22	announced

Dataset with its publication pending

submitter keyword: Candida auris, BCR1, MCU1, skin colonization, metabolic adaptation

Yuchen Deng
contact affiliation	Department of Dermatology, Second Affiliated Hospital of Naval Medical University, Shanghai，200003, China
contact email	smmudeng@outlook.com
lab head
Yuchen Deng
contact affiliation	Fudan University
contact email	smmudeng@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD047167

PRIDE project URI

• PRIDE
  1. PXD047167
     1. Label: PRIDE project
     2. Name: A single gene mutation underpins metabolic adaptation and acquisition of filamentous competence in the emerging fungal pathogen Candida auris