PXD047142 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The Molecular Logic of Gtr1/2 and Pib2 Dependent TORC1 Regulation in Budding Yeast |
| Description | The Target of Rapamycin kinase Complex I (TORC1) regulates cell growth and metabolism in eukaryotes. Previous studies have shown that, in Saccharomyces cerevisiae, nitrogen and amino acid signals activate TORC1 via the highly conserved small GTPases, Gtr1/2, and the phosphatidylinositol 3-phosphate binding protein, Pib2. However, it was unclear if/how Gtr1/2 and Pib2 cooperate to control TORC1. Here we report that this dual regulator system pushes TORC1 into three distinct signaling states: (i) a Gtr1/2 on, Pib2 on, rapid growth state in nutrient replete conditions; (ii) a Gtr1/2 off, Pib2 on, adaptive/slow growth state in poor-quality growth medium; and (iii) a Gtr1/2 off, Pib2 off, quiescent state in starvation conditions. We suggest that other signaling pathways work in a similar way, to drive a multi-level response via a single kinase, but the behavior has been overlooked since most studies follow signaling to a single reporter protein. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-13 |
| AnnouncementXML | Submission_2025-11-13_11:11:06.972.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD047142 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Paul Langlais |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-11-21 16:44:26 | ID requested | |
| ⏵ 1 | 2025-11-13 11:11:07 | announced | |
Publication List
Keyword List
| submitter keyword: Pib2, Saccharomyces cerevisiae, Gtr1, Gtr2,mTORC1 |
Contact List
| Andrew Capaldi |
|---|
| contact affiliation | Associate Professor, Molecular and Cellular Biology Associate Professor, Genetics - GIDP Associate Professor, BIO5 Institute |
| contact email | capaldi@arizona.edu |
| lab head | |
| Paul Langlais |
|---|
| contact affiliation | University of Arizona |
| contact email | langlais@deptofmed.arizona.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047142
- Label: PRIDE project
- Name: The Molecular Logic of Gtr1/2 and Pib2 Dependent TORC1 Regulation in Budding Yeast
