PXD047124-1

PXD047124 is an original dataset announced via ProteomeXchange.

Title	Post-translational regulation of photosynthetic activity via the TOR kinase in plants.
Description	Chloroplasts are the powerhouse of the plant cell, and their activity must be matched to plant growth to avoid photo-oxidative damage. We have identified a post-translational mechanism linking the conserved Target of Rapamycin (TOR) kinase that promotes growth and the guanosine tetraphosphate (ppGpp) signaling pathway that regulates chloroplast activity, and photosynthesis in particular. We find that RelA SpoT Homologue 3 (RSH3), a nuclear-encoded enzyme responsible for ppGpp biosynthesis, interacts directly with the TOR complex via a plant-specific N-terminal region which is phosphorylated in a TOR-dependent manner. Downregulating TOR activity increases ppGpp synthesis by RSH3, and reduces photosynthetic capacity. The TOR-RSH3 signaling axis therefore regulates chloroplast activity to match plant growth, and sets a new precedent for the regulation of organellar function by TOR.
HostingRepository	PRIDE
AnnounceDate	2024-05-03
AnnouncementXML	Submission_2024-05-03_02:35:50.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Regine Lebrun
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	biotinylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-21 06:34:20	ID requested
⏵ 1	2024-05-03 02:35:51	announced
2	2024-10-22 06:38:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: photosynthesis, phosphorylation,TOR kinase, guanosine tetraphosphate signaling, ppGpp

Régine Lebrun
contact affiliation	Régine Lebrun, Aix-Marseille Univ, CNRS, FR3479, Proteomics facility of IMM, Marseille Protéomique, France
contact email	rlebrun@imm.cnrs.fr
lab head
Regine Lebrun
contact affiliation	CNRS
contact email	rlebrun@imm.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047124
     1. Label: PRIDE project
     2. Name: Post-translational regulation of photosynthetic activity via the TOR kinase in plants.