PXD047087-1

PXD047087 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comprehensive identification of Ubiquitin-like 3 (UBL3)-interacting proteins in mouse brain
Description	Each protein extract from the cortex of Ubl3+/+ and Ubl3Tg/+ male mice (9-10-week-old, n=3, 3) was incubated with 30 μL of NeutrAvidin Agarose (ThermoFisher, 29202) for 18 h at 4 °C and subjected to mass spectrometry using previously reported methods (Ageta et al., Nat Commun 2018, PMID: 30258067). The peptides were analyzed by liquid chromatography/mass spectrometry (LC/MS) using an Orbitrap Fusion mass spectrometer coupled to an Easy-nLC 1000 using a EASY-Spray ES900 column system (ThermoFisher, 75 μm x 150 mm, particle size 3 μm). Mass spectrometry raw files is proceeded using Proteome Discoverer version 2.4 (ThermoFisher) and a local MASCOT server (version 2.6.2; Matrix Science). MS/MS data were searched against Mus musculus (SwissProt TaxID=10090_and_subtaxonomies) (v2017-10-25).
HostingRepository	jPOST
AnnounceDate	2024-11-20
AnnouncementXML	Submission_2024-11-19_07:00:07.317.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047087
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hiroshi Ageta
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-20 01:22:03	ID requested
⏵ 1	2024-11-19 07:00:07	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: UBL3

submitter keyword: UBL3

Contact List

Kunihiro Tsuchida
lab head
Hiroshi Ageta
contact affiliation	Fujita Health University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002396/