PXD047012-1
PXD047012 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Loss of adipocyte-specific Olfactomedin-2 in obesity drives defective fat cell function, aggravating adipose tissue accretion |
| Description | Olfactomedin-2 (OLFM2) participates in brain development and appears to be involved in energy handling. In adipose tissue, we here show expression of OLFM2 to be adipocyte-specific and opposed to obesity. OLFM2 levels are increased during adipogenesis, and impaired when differentiated fat cells are challenged with conditions emulating inflammation in the context of obesity. On the molecular level, examination of OLFM2 deficiency in human adipocytes indicated down-regulation of genes related to cell cycle. At the cellular level, the loss of OLFM2 compromised adipogenesis, while its over-production enhanced the adipogenic transformation of 3T3-L1 cells. Complementary loss and gain of function assays coupled to untargeted proteomics revealed the modulation of key protein pathways, including regulation of citrate cycle, fatty acid degradation, axon guidance and focal adhesion in mature adipocytes and precursor cells. Transferring these findings into animal models using a whole-body knockout and transcriptionally depleted adipose Olfm2 highlighted, respectively, a cluster of molecular changes connected with defective cell cycle (in both), fat mass accretion and impaired metabolism (in the latter). Our data underscores a key role for OLFM2 in fat cells, and suggests that the association between adipose OLFM2 and obesity is not only correlative but also causative. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-11-17 |
| AnnouncementXML | Submission_2023-11-17_01:26:36.878.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Witold Szymanski |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Deamidated:18O(1); Acetyl |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-16 02:28:30 | ID requested | |
| ⏵ 1 | 2023-11-17 01:26:37 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Olfactomedin-2, adipocytes, adipose tissue, metabolism, obesity, label-free, Orbitrap Eclipse, DIA |
Contact List
| Francisco J. Ortega, Ph.D. | |
|---|---|
| contact affiliation | Institut d'Investigacio Biomedica de Girona (IDIBGI) |
| contact email | fortega@idibgi.org |
| lab head | |
| Witold Szymanski | |
| contact affiliation | Philipps-University Marburg Biochemical/Pharmacological Center Department of Medicine |
| contact email | witold.szymanski@uni-marburg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093409/ |
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