PXD047009-1

PXD047009 is an original dataset announced via ProteomeXchange.

Title	Exosomes define a local and systemic communication network in healthy pancreas and in pancreatic ductal adenocarcinoma
Description	Pancreatic ductal adenocarcinoma (PDAC) is a deadly disease. Understanding its biology is crucial for finding effective therapies. Exosomes have been implicated in cancer, but their behavior in living systems remains poorly understood. We aimed to map the spatiotemporal distribution of exosomes from both healthy pancreas and PDAC to determine their biological significance. To achieve this, we developed a genetically engineered mouse model (ExoBow) to trace spontaneous exosomes communication. Within the PDAC microenvironment, cancer cells establish preferential communication routes with cancer associated fibroblasts and endothelial cells. The latter is a conserved event in the healthy pancreas. Inhibiting exosomes secretion in both scenarios significantly enhanced angiogenesis, underscoring the contribution of exosomes to the vascularization of the organ and to cancer. Inter-organ communication is significantly increased in PDAC, and the thymus is a sustained target in both contexts. PDAC cells also communicate with the kidneys and lungs, and the healthy pancreas with bone-marrow, brain, and intestines. In sum, we found that exosomes mediate an organized communication network in vivo that controls angiogenesis locally, both in the healthy pancreas and PDAC, and that targets the thymus also in both conditions, unravelling their potential role in central immune surveillance and anti-tumor immune response.
HostingRepository	PRIDE
AnnounceDate	2023-12-12
AnnouncementXML	Submission_2023-12-12_10:47:12.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-16 00:40:48	ID requested
⏵ 1	2023-12-12 10:47:13	announced
2	2024-10-22 06:17:22	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: exosomes,Pancreatic cancer, intercellular communication, tumor microenvironment

Sonia A. Melo
contact affiliation	i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. Departamento de Patologia, Faculdade de Medicina, Universidade do Porto, Portugal. P.CCC Porto Comprehensive Cancer Center Raquel Seruca, Portugal.
contact email	smelo@i3s.up.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047009

PRIDE project URI

• PRIDE
  1. PXD047009
     1. Label: PRIDE project
     2. Name: Exosomes define a local and systemic communication network in healthy pancreas and in pancreatic ductal adenocarcinoma