PXD046988-1

PXD046988 is an original dataset announced via ProteomeXchange.

Title	SOURCES OF BIASES IN THE IN VITRO TESTING OF NANOMATERIALS: THE ROLE OF BIOMOLECULAR CORONA
Description	The biological fate of nanomaterials (NMs) is driven by the specific interactions that biomolecules, naturally adhering onto their surface, engage with cell membrane receptors and intracellular organelles. The molecular composition of this layer, called biomolecular corona (BMC), depends on both the physical-chemical features of the NM and the biological media in which the NM is dispersed and cells grow. In this work, we demonstrate that the widespread use of 10% fetal bovine serum for in vitro assay is unable to recapitulate the complexity of an in vivo systemic administration, with NMs being transported by the blood. To this purpose, we undertook a comparative journey involving proteomics, lipidomics, high-throughput multiparametric in vitro screening, as well as single molecular feature analysis, to investigate the molecular details behind this in vivo/in vitro bias. Our work indirectly highlights the need to introduce novel, more physiological-like media, closer in composition to human plasma, to produce realistic in vitro screening data for NMs. We also aim to set the basis to reduce this in vitro-in vivo mismatch, which currently limits the formulation of NMs for clinical settings.
HostingRepository	PRIDE
AnnounceDate	2024-05-31
AnnouncementXML	Submission_2024-05-31_03:35:57.880.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Armirotti
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-15 06:16:07	ID requested
⏵ 1	2024-05-31 03:35:58	announced
2	2024-10-22 06:43:29	announced	2024-10-22: Updated project metadata.

10.1039/D3NH00510K;

submitter keyword: graphene, gold nanoparticles, biomolecular corona,Nanomaterials

Andrea Armirotti
contact affiliation	Analytical Chemistry Facility - Istituto Italiano di Tecnologia, Italy
contact email	andrea.armirotti@iit.it
lab head
Andrea Armirotti
contact affiliation	Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia
contact email	andrea.armirotti@iit.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046988

PRIDE project URI

• PRIDE
  1. PXD046988
     1. Label: PRIDE project
     2. Name: SOURCES OF BIASES IN THE IN VITRO TESTING OF NANOMATERIALS: THE ROLE OF BIOMOLECULAR CORONA