PXD046971-1

PXD046971 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation regulates viral biomolecular condensates to promote infectious progeny production
Description	Biomolecular condensates play important roles in diverse biological processes. Many viruses form biomolecular condensates which have been implicated in various functions critical for productive infection of host cells. The adenovirus L1-52/55 kilodalton protein (52K) was recently shown to form viral biomolecular condensates that coordinate viral genome packaging and capsid assembly. Although critical for packaging, we do not know how viral condensates are regulated during adenovirus infection. Here we show that phosphorylation of serine residues 28 and 75 within the N-terminal intrinsically disordered region of 52K modulates viral condensates in vitro and in cells, promoting liquid-like properties over condensate hardening. Furthermore, we demonstrate that phosphorylation of 52K promotes viral genome packaging and production of infectious progeny particles. Collectively, our findings provide insights into how viral condensate properties are regulated and maintained in a state conducive to their function in viral progeny production. In addition, our findings have implications for antiviral strategies aimed at targeting the regulation of viral biomolecular condensates to limit viral multiplication.
HostingRepository	PRIDE
AnnounceDate	2023-12-06
AnnouncementXML	Submission_2023-12-06_14:41:02.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Lauman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 18:32:27	ID requested
⏵ 1	2023-12-06 14:41:02	announced
2	2024-10-22 06:16:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Biomolecular Condensates,Phosphorylation, Virus

Richard Lauman
contact affiliation	Childrens Hospital of Pennsylvania University of Pennsylvania
contact email	richard.lauman@gmail.com
lab head
Richard Lauman
contact affiliation	Universtiy of Pennsylvania
contact email	richard.lauman@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046971
     1. Label: PRIDE project
     2. Name: Phosphorylation regulates viral biomolecular condensates to promote infectious progeny production