PXD046959-1

PXD046959 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Illuminating phenotypic drug responses of sarcoma cells to kinase inhibitors by phosphoproteomics-2
Description	Kinase inhibitors (KIs) are important cancer drugs but often display polypharmacology that is molecularly not understood. This disconnect is particularly apparent in cancer entities such as sarcomas for which the oncogenic drivers are often not clear. To investigate more systematically how the cellular proteotypes of sarcoma cells shape their response to molecularly targeted drugs, we profiled the proteomes and phosphoproteomes of 17 sarcoma cell lines and screened the same cells against 150 cancer drugs. The resulting 2,550 phenotypic drug profiles revealed distinct drug responses and the cellular activity landscapes derived from deep (phospho)proteomes (9-10,000 proteins and 10-27,000 phosphorylation sites per cell line) enabled several lines of analysis. For instance, connecting the (phospho)proteomic data with drug responses revealed known and novel mechanisms of action (MoAs) of kinase inhibitors and identified markers of drug sensitivity or resistance. All data is publically accessible via an interactive web application that enables exploration of this rich molecular resource for better understanding active signalling pathways in sarcoma cells, identifying treatment response predictors, and revealing novel MoA of clinical KIs.
HostingRepository	MassIVE
AnnounceDate	2023-11-29
AnnouncementXML	Submission_2023-11-29_10:49:50.353.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chien-Yun Lee
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	TMT6plex; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 14:17:41	ID requested
⏵ 1	2023-11-29 10:49:50	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: phosphoproteomics, kinase inhibitor, sarcoma, drug response, proteotype

submitter keyword: phosphoproteomics, kinase inhibitor, sarcoma, drug response, proteotype

Contact List

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, School of Life Sciences, Technical University of Munich
contact email	kuster@tum.de
lab head
Chien-Yun Lee
contact affiliation	Technical University of Munich
contact email	chienyun.lee@tum.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093533/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093533/