PXD046937-1

PXD046937 is an original dataset announced via ProteomeXchange.

Title	iTRAQ based disease responsive extracellular matrix proteome of jute infected with Macrophomina Phaseolina
Description	Extracellular matrix (ECM) is the first line of defense which is an inimitable organelle that perceives biotic and abiotic stresses and reprograms biological processes of host. It also activates innate immune responses in temporal and spatial manner and acts as physical scaffold that prevents the entry of pathogen and microbes in response to stress and hormonal signals. Stem rot, caused by Macrophomina phaseolina adversely affects fiber production in jute. However, how wall related susceptibility affects ECM proteome remains undetermined in bast fiber crops. Here, stem rot responsive quantitative temporal ECM proteome was developed in jute upon M. phaseolina infection. Using isobaric tags for relative and absolute quantitative proteomics and MS/MS analysis, we identified 415 disease responsive proteins (DRPs), involved in wall integrity, acidification, proteostasis, hydration and redox homeostasis. Disease-related correlation network identified functional hubs related to wall degrading enzymes, structural carbohydrates and signaling govern rot responsive wall-susceptibility.
HostingRepository	PRIDE
AnnounceDate	2024-05-21
AnnouncementXML	Submission_2024-05-21_09:32:14.287.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Subhra Chakraborty
SpeciesList	scientific name: Corchorus olitorius; NCBI TaxID: 93759;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 01:09:22	ID requested
⏵ 1	2024-05-21 09:32:15	announced

10.1021/acs.jproteome.3c00781;

Arafat MY, Narula K, Kumar M, Chakraborty N, Chakraborty S, Proteo-metabolomic Dissection of Extracellular Matrix Reveals Alterations in Cell Wall Integrity and Calcium Signaling Governs Wall-Associated Susceptibility during Stem Rot Disease in Jute. J Proteome Res, 23(8):3217-3234(2024) [pubmed]

submitter keyword: mass spectrometry, stem rot disease,Jute, extracellular matrix

Dr. Subhra Chakraborty
contact affiliation	National Institute for Plant Genome Research Aruna Asaf Ali Marg New Delhi - 110 067
contact email	subhrac@hotmail.com
lab head
Subhra Chakraborty
contact affiliation	National Institute of Plant Genome Research
contact email	subhrac@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046937

PRIDE project URI

• PRIDE
  1. PXD046937
     1. Label: PRIDE project
     2. Name: iTRAQ based disease responsive extracellular matrix proteome of jute infected with Macrophomina Phaseolina