PXD046918-1
PXD046918 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa versus Single Exposure through Photosynthesis, Oxidative Stress and Energy Metabolism shift |
Description | Antibiotics are increasingly detected in aquatic environments, and their potential ecological risk is a concern. However, most antibiotic toxicity studies were performed with single exposure experiments. Here, we studied the effects and mechanisms of repeated clarithromycin (CLA) exposure on the algae Microcystis aeruginosa compared to the results of a single exposure. The 96-h effective concentration of CLA was 13.37 μg/L upon single exposure but was reduced to 6.90 μg/L upon repeated exposure. Single-exposure CLA inhibited algal photosynthesis by disrupting energy absorption, dissipation and trapping, reaction center activation, and electron transport, thereby inducing oxidative stress and ultrastructural damage. CLA also upregulated glycolysis, pyruvate metabolism, and the tricarboxylic acid cycle. Stronger inhibition of algal growth was observed under repeated exposure via effects on photosynthetic pigments, biosynthesis of reaction center subunits, and electron transport, inducing more substantial oxidative damage. Furthermore, repeated exposure reduced carbohydrate utilization by blocking the pentose phosphate pathway, which changed the characteristics of extracellular polymeric substances and eventually impaired algal self-defense. Risk quotients calculated from repeated exposure were higher than 1, indicating significant ecological risks. This study elucidated the severe influence of repeated antibiotic exposure on algae, providing new insights into antibiotic risk assessment. |
HostingRepository | iProX |
AnnounceDate | 2023-11-13 |
AnnouncementXML | Submission_2023-11-13_19:43:21.026.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kailai Ye |
SpeciesList | scientific name: Microcystis aeruginosa G-01; NCBI TaxID: 1009696; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-13 19:43:03 | ID requested | |
⏵ 1 | 2023-11-13 19:43:21 | announced | |
2 | 2024-05-20 20:13:38 | announced | 2024-05-21: Update information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Microcystis aeruginosa, Clarithromycin, Repeated exposure, Metabolomics, Ecological risk |
Contact List
Jiang Hu | |
---|---|
contact affiliation | Key Laboratory of Hydraulic and Waterway Engineering, Ministry of Education, Chongqing Jiaotong University |
contact email | 378922494@qq.com |
lab head | |
Kailai Ye | |
contact affiliation | Key Laboratory of Hydraulic and Waterway Engineering, Ministry of Education, Chongqing Jiaotong University, Chongqing 400074, China |
contact email | 1545499257@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |