PXD046906 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Trypanosoma cruzi interaction with host tissues modulate the composition of large extracellular vesicles |
| Description | Trypanosoma cruzi is the protozoan that causes Chagas disease, an endemic parasitosis in Latin America that has spread around the globe. Recently, a series of studies indicate that the gastrointestinal tract represents an important reservoir for T. cruzi in the chronic phase. It is also known that, during contact between T. cruzi and host cells, there is a release of extracellular vesicles (EVs) that modulates the immune system and enhances the infection, but the dynamics of secretion of host and parasite molecules through these EVs is not understood. In this study, we used two cell lines to simulate the environments found by the parasite in the host: C2C12 cell (myoblast) and Caco-2 cell (intestinal epithelium). We isolated large EVs (LEVs) from the interaction of T. cruzi culture-derived trypomastigotes (TCTs) belonging to two distinct strains (CL Brener, DTU Tc VI and Dm28c DTU Tc I) in contact with C2C12 and Caco-2 cells to 2 hours and after 24 hours of infection. The interaction of the parasite with the host cell induces a switch in the functionality of proteins carried by LEVs and a varied tissue answer. Protein-protein interaction analysis indicates that LEVs carry key proteins for host-pathogen interaction that could participate in the pathogenesis of Chagas Disease. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_06:39:40.515.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rafael de Almeida |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333; scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-11-13 07:20:43 | ID requested | |
| 1 | 2024-05-17 10:05:18 | announced | |
| ⏵ 2 | 2024-10-22 06:39:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: mass spectrometry, tropism, microvesicles, extracellular vesicles,Trypanosoma cruzi |
Contact List
| Marcel Ivan |
|---|
| contact affiliation | EVAHPI Research Group, Laboratório de Biologia Celular, Instituto Carlos Chagas, Fundação Oswaldo Cruz |
| contact email | marcel.ivan.ramirez@gmail.com |
| lab head | |
| Rafael de Almeida |
|---|
| contact affiliation | Fiocruz |
| contact email | alosteria@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046906 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046906
- Label: PRIDE project
- Name: Trypanosoma cruzi interaction with host tissues modulate the composition of large extracellular vesicles
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