PXD046853 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MSMS of the MHC-I immunopeptidome of AML cell line, THP-1 |
Description | The hypomethylating agent 5-azacytidine (AZA) is the first-line induction therapy for AML patients unsuitable for intensive chemotherapy. The anti-tumor effect of AZA results in part from T-cell cytotoxic responses against MHC-I-associated peptides (MAPs) deriving from hypermethylated genomic regions such as cancer-testis antigens (CTAs), or endogenous retroelements (EREs). However, clear evidence supporting higher ERE MAPs presentation after AZA treatment in AML is lacking. Interestingly, we find that AZA-mediated DNMT2 inhibition leads to autophagy induction, which is responsible for mitigating ERE MAPs generation. To validate our findings, we examined the immunopeptidome of THP-1 cells treated with AZA combined with autophagy inhibitor, Spautin-1 or decitabine (DAC, non-DNMT2 inhibiting HMA) through a proteogenomic approach. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-17 |
AnnouncementXML | Submission_2024-04-17_06:02:33.286.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD046853 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Courcelles Mathieu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Ascend |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-10 10:37:29 | ID requested | |
⏵ 1 | 2024-04-17 06:02:33 | announced | |
Publication List
Keyword List
submitter keyword: Hypomethylating agents (HMAs), acute myeloid leukemia, EREs, autophagy inhibition, immunopeptidome, MHC-I |
Contact List
Pierre Thibault |
contact affiliation | Institute for Research in Immunology and Cancer, Department of Biochemistry, Department of Chemistry, Université de Montréal, Québec, Canada H3T 1J4 |
contact email | pierre.thibault@umontreal.ca |
lab head | |
Courcelles Mathieu |
contact affiliation | IRIC |
contact email | mathieu.courcelles@umontreal.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046853
- Label: PRIDE project
- Name: LC-MSMS of the MHC-I immunopeptidome of AML cell line, THP-1