PXD046826-1
PXD046826 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multiomics characterization of a less invasive microfluidic-based cell sorting technique |
| Description | Fluorescence-activated cell sorting (FACS) is a specialized technique to isolate cell subpopulations with a high level of recovery and accuracy. However, the cell sorting procedure can impact the viability and metabolic state of cells. Here, we performed a comparative study and evaluated the impact of traditional high-pressure charged droplet-based and a microfluidic chip-based sorting approach on the metabolic and phosphoproteomic profile of different cell types. While microfluidic chip-based sorted cells more closely resembled the unsorted control group for most cell types tested, the droplet-based sorted cells showed significant metabolic and phosphoproteomic alterations. In particular, greater changes in redox and energy status were present in cells sorted with the droplet-based cell sorter along with higher transcriptional and spliceosomal regulation and mechanical stress signaling. These results indicate microfluidic chip-based sorting is less disruptive compared to droplet-based sorting. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-19 |
| AnnouncementXML | Submission_2024-03-19_12:49:26.829.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jana Zecha |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Acetyl; Phospho; Carbamidomethyl |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-09 13:53:18 | ID requested | |
| ⏵ 1 | 2024-03-19 12:49:27 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Sorter-induced cellular stress (SICS), Phosphoproteomics, Fluorescence-activated cell sorting (FACS), Sorting |
Contact List
| Sonja Hess | |
|---|---|
| contact affiliation | AstraZeneca |
| contact email | sonja.hess@astrazeneca.com |
| lab head | |
| Jana Zecha | |
| contact affiliation | AstraZeneca |
| contact email | jana.zecha@astrazeneca.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093348/ |
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