PXD046812-1

PXD046812 is an original dataset announced via ProteomeXchange.

Title	Cortical layer 5 neuron-specific synaptic protein profiling suggests differential vulnerability to neurodevelopmental disorders
Description	Pyramidal neurons in the cortex are embedded in distinct information processing pathways. Cortical layer 5 (L5) intratelencephalic (IT) and pyramidal tract (PT) neurons receive different input and project to distinct brain regions. The synaptic molecular signatures that define synaptic connectivity and function of L5 IT and PT neurons are largely unknown. Here, we use an optimized proximity biotinylation workflow to characterize the excitatory postsynaptic proteomes of L5 IT and PT neurons in intact somatosensory circuits. We find that differential expression of neurotransmitter receptors, ion channels and cell-surface proteins (CSPs), most prominently of the leucine-rich repeat (LRR) family, specifies L5 IT and PT neuron input connectivity and function. Our analysis further uncovers differential vulnerability to neurodevelopmental disorders for L5 IT and PT neurons, with a marked enrichment of autism risk genes in the postsynaptic excitatory proteome of IT, but not of PT, neurons. Together, cell type- and input type-specific synaptic proteome profiling implies that many of the proteins specifying connectivity and function of two closely related cortical pyramidal cell types also underlie their differential vulnerability to neurodevelopmental disorders.
HostingRepository	PRIDE
AnnounceDate	2024-11-04
AnnouncementXML	Submission_2024-11-04_13:24:55.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-09 03:06:03	ID requested
⏵ 1	2024-11-04 13:24:55	announced

Dataset with its publication pending

submitter keyword: synaptic proteome,Neurodevelopment, turboID

Professor Joris de Wit
contact affiliation	VIB Center for Brain & Disease Research, Herestraat 49, 3000 Leuven, Belgium.
contact email	joris.dewit@kuleuven.be
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046812
     1. Label: PRIDE project
     2. Name: Cortical layer 5 neuron-specific synaptic protein profiling suggests differential vulnerability to neurodevelopmental disorders