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PXD046811-1

PXD046811 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDevelopment of a Global Metabo-Lipid-Prote-omics Workflow to Compare Healthy Proximal and Distal Colonic Epithelium in Mice
DescriptionA multimetabo-lipid-prote-omics workflow was developed to characterize the molecular interplay within proximal (PC) and distal (DC) colonic epithelium of healthy mice. This multiomics data set lays the foundation to better understand the two tissue types and can be used to study, for example, colon-related diseases like colorectal cancer or inflammatory bowel disease. First, the methyl tert-butyl ether extraction method was optimized, so that from a single tissue biopsy >350 reference-matched metabolites, >1850 reference-matched lipids, and >4500 proteins were detected by using targeted and untargeted metabolomics, untargeted lipidomics, and proteomics. Next, each omics-data set was analyzed individually and then merged with the additional omics disciplines to generate a deep understanding of the underlying complex regulatory network within the colon. Our data demonstrates, for example, differences in mucin formation, detected on substrate level as well as on enzyme level, and altered lipid metabolism by the detection of phospholipases hydrolyzing sphingomyelins to ceramides. In conclusion, the combination of the three mass spectrometry-based omics techniques can better entangle the functional and regional differences between PC and DC tissue compared to each single omics technique.
HostingRepositoryPRIDE
AnnounceDate2024-09-04
AnnouncementXMLSubmission_2024-09-04_04:35:54.561.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristina Ludwig
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-09 02:49:17ID requested
12024-09-04 04:35:55announced
22024-10-22 06:57:44announced2024-10-22: Updated project metadata.
Publication List
Hemmati M, Wudy SI, Hackbarth F, Mittermeier-Kle, ß, inger VK, Coleman OI, Haller D, Ludwig C, Dawid C, Kleigrewe K, Development of a Global Metabo-Lipid-Prote-omics Workflow to Compare Healthy Proximal and Distal Colonic Epithelium in Mice. J Proteome Res, 23(8):3124-3140(2024) [pubmed]
10.1021/acs.jproteome.3c00771;
Keyword List
submitter keyword: Metabo-lipid-proteomics
Methyl tert-butyl ether
Mouse proximal and distal colons
multi-omics
Contact List
Christina Ludwig
contact affiliationBavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technical University Munich (TUM) Gregor-Mendel-Strasse 4 85354 Freising GERMANY
contact emailtina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliationTU Munich
contact emailtina.ludwig@tum.de
dataset submitter
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Dataset FTP location
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