PXD046804 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Facile metabolic reprogramming distinguishes mycobacterial adaptation to hypoxia and starvation: The role of ketosis in starvation-induced persistence |
Description | Mycobacteria have the ability to adapt to stressful infection conditions by entering a reversible state of non-replicating persistence (NRP) characterized by slow or no cell growth and increased tolerance to various antimicrobial agents. While hypoxia and nutrient deprivation are commonly used to study this NRP state, there is limited understanding of the molecular distinctions in how mycobacteria adapt to these different stresses to achieve a similar NRP outcome. In this study, we conducted a comprehensive analysis of Mycobacterium bovis BCG's response to starvation, shedding light on a coordinated metabolic shift away from glycolysis during nutrient-rich growth to the depletion of lipid stores, lipolysis, and fatty acid ß-oxidation during NRP. Interestingly, this response differs from BCG's NRP state under hypoxia, where it shifts towards cholesterol metabolism and triglyceride storage. Our investigation also unveiled hidden metabolic vulnerabilities in the NRP state induced by starvation, notably an increased sensitivity to H2O2. These findings open up possibilities for the development of precise therapeutic approaches against these otherwise challenging-to-treat pathogens. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:39:04.510.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fabian Hia |
SpeciesList | scientific name: Mycobacterium bovis BCG str. Pasteur 1173P2; NCBI TaxID: 410289; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap; 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-08 22:00:57 | ID requested | |
⏵ 1 | 2024-10-08 11:39:04 | announced | |
2 | 2024-10-22 06:13:49 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mycobacterium BCG starvation proteomics |
Contact List
Peter Dedon |
contact affiliation | Singapore Professor of Biological Engineering Professor of Biological Engineering Massachusetts Institute of Technology Department of Biological Engineering, 56-731 77 Massachusetts Ave. Cambridge, MA 02139 |
contact email | pcdedon@mit.edu |
lab head | |
Fabian Hia |
contact affiliation | Singapore-MIT Alliance for Reseach and Technology |
contact email | fabian.hia@smart.mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046804
- Label: PRIDE project
- Name: Facile metabolic reprogramming distinguishes mycobacterial adaptation to hypoxia and starvation: The role of ketosis in starvation-induced persistence