PXD046804-1

PXD046804 is an original dataset announced via ProteomeXchange.

Title	Facile metabolic reprogramming distinguishes mycobacterial adaptation to hypoxia and starvation: The role of ketosis in starvation-induced persistence
Description	Mycobacteria have the ability to adapt to stressful infection conditions by entering a reversible state of non-replicating persistence (NRP) characterized by slow or no cell growth and increased tolerance to various antimicrobial agents. While hypoxia and nutrient deprivation are commonly used to study this NRP state, there is limited understanding of the molecular distinctions in how mycobacteria adapt to these different stresses to achieve a similar NRP outcome. In this study, we conducted a comprehensive analysis of Mycobacterium bovis BCG's response to starvation, shedding light on a coordinated metabolic shift away from glycolysis during nutrient-rich growth to the depletion of lipid stores, lipolysis, and fatty acid ß-oxidation during NRP. Interestingly, this response differs from BCG's NRP state under hypoxia, where it shifts towards cholesterol metabolism and triglyceride storage. Our investigation also unveiled hidden metabolic vulnerabilities in the NRP state induced by starvation, notably an increased sensitivity to H2O2. These findings open up possibilities for the development of precise therapeutic approaches against these otherwise challenging-to-treat pathogens.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:39:04.510.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Hia
SpeciesList	scientific name: Mycobacterium bovis BCG str. Pasteur 1173P2; NCBI TaxID: 410289;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap; 6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-08 22:00:57	ID requested
⏵ 1	2024-10-08 11:39:04	announced
2	2024-10-22 06:13:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mycobacterium BCG starvation proteomics

Peter Dedon
contact affiliation	Singapore Professor of Biological Engineering Professor of Biological Engineering Massachusetts Institute of Technology Department of Biological Engineering, 56-731 77 Massachusetts Ave. Cambridge, MA 02139
contact email	pcdedon@mit.edu
lab head
Fabian Hia
contact affiliation	Singapore-MIT Alliance for Reseach and Technology
contact email	fabian.hia@smart.mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046804
     1. Label: PRIDE project
     2. Name: Facile metabolic reprogramming distinguishes mycobacterial adaptation to hypoxia and starvation: The role of ketosis in starvation-induced persistence