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PXD046797-1

PXD046797 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity in the rat liver.
DescriptionTo identify the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity we purified subcellular organelles including cytosol, late endosomes (LE/MVBs) and lysosomes (CMA+) from the rat liver and performed co-Ip experiment with antibodies directed against the major cellular chaperones mediating CMA and eMI. As such, we analyzed Hsc70-interacting proteins in LE/MVB, CMA+ lysosomes and cytosol by performing pull-down experiments (co-IP) using anti-Hsc70 antibodies. A comparative analysis of the Hsc-70 interactomes revealed that proteins bound to Hsc70 in cytosol and LE/MVBs or in cytosol and CMA+ lysosomes were considered eMI or CMA substrates, respectively.
HostingRepositoryPRIDE
AnnounceDate2024-01-26
AnnouncementXMLSubmission_2024-01-26_07:45:33.115.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD046797
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCristina Clement
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListdeamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-08 16:37:26ID requested
12024-01-26 07:45:33announced
22024-10-22 06:26:30announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2023.113529;
10.6019/PXD046797;
Krause GJ, Kirchner P, Stiller B, Morozova K, Diaz A, Chen KH, Krogan NJ, Agullo-Pascual E, Clement CC, Lindenau K, Swaney DL, Dilipkumar S, Bravo-Cordero JJ, Santambrogio L, Cuervo AM, Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy. Cell Rep, 42(12):113529(2023) [pubmed]
Keyword List
submitter keyword: CMA vs microautophagy
HSc-70-CO-IP-MS/MS in late endosomes,rat liver, lysosomes and cytosol
Contact List
Anna-Maria Cuervo
contact affiliationDepartment of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
contact emailana-maria.cuervo@einsteinmed.edu
lab head
Cristina Clement
contact affiliationWeill Cornell Medicine
contact emailccc4002@med.cornell.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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