PXD046797 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity in the rat liver. |
Description | To identify the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity we purified subcellular organelles including cytosol, late endosomes (LE/MVBs) and lysosomes (CMA+) from the rat liver and performed co-Ip experiment with antibodies directed against the major cellular chaperones mediating CMA and eMI. As such, we analyzed Hsc70-interacting proteins in LE/MVB, CMA+ lysosomes and cytosol by performing pull-down experiments (co-IP) using anti-Hsc70 antibodies. A comparative analysis of the Hsc-70 interactomes revealed that proteins bound to Hsc70 in cytosol and LE/MVBs or in cytosol and CMA+ lysosomes were considered eMI or CMA substrates, respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_07:45:33.115.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD046797 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Cristina Clement |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-08 16:37:26 | ID requested | |
⏵ 1 | 2024-01-26 07:45:33 | announced | |
2 | 2024-10-22 06:26:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.celrep.2023.113529; |
10.6019/PXD046797; |
Krause GJ, Kirchner P, Stiller B, Morozova K, Diaz A, Chen KH, Krogan NJ, Agullo-Pascual E, Clement CC, Lindenau K, Swaney DL, Dilipkumar S, Bravo-Cordero JJ, Santambrogio L, Cuervo AM, Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy. Cell Rep, 42(12):113529(2023) [pubmed] |
Keyword List
submitter keyword: CMA vs microautophagy |
HSc-70-CO-IP-MS/MS in late endosomes,rat liver, lysosomes and cytosol |
Contact List
Anna-Maria Cuervo |
contact affiliation | Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. |
contact email | ana-maria.cuervo@einsteinmed.edu |
lab head | |
Cristina Clement |
contact affiliation | Weill Cornell Medicine |
contact email | ccc4002@med.cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046797 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046797
- Label: PRIDE project
- Name: Identification of the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity in the rat liver.