PXD046797-1

PXD046797 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity in the rat liver.
Description	To identify the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity we purified subcellular organelles including cytosol, late endosomes (LE/MVBs) and lysosomes (CMA+) from the rat liver and performed co-Ip experiment with antibodies directed against the major cellular chaperones mediating CMA and eMI. As such, we analyzed Hsc70-interacting proteins in LE/MVB, CMA+ lysosomes and cytosol by performing pull-down experiments (co-IP) using anti-Hsc70 antibodies. A comparative analysis of the Hsc-70 interactomes revealed that proteins bound to Hsc70 in cytosol and LE/MVBs or in cytosol and CMA+ lysosomes were considered eMI or CMA substrates, respectively.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:45:33.115.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046797
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cristina Clement
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-08 16:37:26	ID requested
⏵ 1	2024-01-26 07:45:33	announced
2	2024-10-22 06:26:30	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/j.celrep.2023.113529;
10.6019/PXD046797;
Krause GJ, Kirchner P, Stiller B, Morozova K, Diaz A, Chen KH, Krogan NJ, Agullo-Pascual E, Clement CC, Lindenau K, Swaney DL, Dilipkumar S, Bravo-Cordero JJ, Santambrogio L, Cuervo AM, Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy. Cell Rep, 42(12):113529(2023) [pubmed]

10.1016/j.celrep.2023.113529;

10.6019/PXD046797;

Krause GJ, Kirchner P, Stiller B, Morozova K, Diaz A, Chen KH, Krogan NJ, Agullo-Pascual E, Clement CC, Lindenau K, Swaney DL, Dilipkumar S, Bravo-Cordero JJ, Santambrogio L, Cuervo AM, Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy. Cell Rep, 42(12):113529(2023) [pubmed]

Keyword List

submitter keyword: CMA vs microautophagy
HSc-70-CO-IP-MS/MS in late endosomes,rat liver, lysosomes and cytosol

submitter keyword: CMA vs microautophagy

HSc-70-CO-IP-MS/MS in late endosomes,rat liver, lysosomes and cytosol

Contact List

Anna-Maria Cuervo
contact affiliation	Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
contact email	ana-maria.cuervo@einsteinmed.edu
lab head
Cristina Clement
contact affiliation	Weill Cornell Medicine
contact email	ccc4002@med.cornell.edu
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046797

PRIDE project URI

Repository Record List

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