PXD046794-1

PXD046794 is an original dataset announced via ProteomeXchange.

Title	Fine mapping of the pulmonary artery matrisome in a large animal model of hypoxia-induced pulmonary hypertension
Description	Pulmonary hypertension (PH) is a progressive disease with a high mortality rate, usually as a result of right heart failure. The pulmonary vasculature in PH is characterized by remodeling and stiffening, potentially with luminal obstruction, driven by enhanced cell proliferation/survival versus cell death, chronic inflammation, and altered extracellular matrix (ECM), with an increase in vascular fibrosis. Given the poorly understood biochemical nature of pulmonary vascular remodeling, we evaluated the ECM and ECM-associated protein composition of the main pulmonary artery (MPA), distal pulmonary arteries (DPA) distal whole lung (DWL) of acute hypoxia-driven PH (in a young bovine calf model) using high-resolution mass spectrometry. Significant differences in the proteomic signatures were observed in the three vascular/tissue sites. Alterations included a strong immune response and wound repair signature including several proteins from the coagulation cascade and markers of a provisional matrix. These findings support a role for disease propagation that proceeds through a wound-healing trajectory with a strong hemostasis and provision matrix in the MPA, to a more destructive remodeling with higher turnover in the most distal capillaries. This data provides insight into short-term remodeling that results in robust biomechanical manifestation of PH and initiates a resolvable disease trajectory when hypoxic insult is removed.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_20:55:46.913.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kirk Hansen
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-08 14:37:29	ID requested
⏵ 1	2025-12-07 20:55:47	announced

10.1152/ajpcell.00274.2024;

Williams J, Iheagwam FN, Maroney SP, Schmitt LR, Brown RD, Krafsur GM, Frid MG, McCabe MC, Gandjeva A, Williams KJ, Luyendyk JP, Saviola AJ, Tuder RM, Stenmark K, Hansen KC, A bovine model of hypoxia-induced pulmonary hypertension reveals a gradient of immune and matrisome response with a complement signature found in circulation. Am J Physiol Cell Physiol, 327(6):C1666-C1680(2024) [pubmed]

submitter keyword: intravascular fibrin, proteomics, intima, pulmonary artery, matrisome, fibrosis,Pulmonary hypertension, extracellular matrix, blood vessel wall

Kirk Hansen
contact affiliation	Department of Biochemistry and Molecular Genetics, University of Colorado, Aurora, CO 80045
contact email	kirk.hansen@cuanschutz.edu
lab head
Kirk Hansen
contact affiliation	Univeristy of Colorado
contact email	kirk.hansen@cuanschutz.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046794
     1. Label: PRIDE project
     2. Name: Fine mapping of the pulmonary artery matrisome in a large animal model of hypoxia-induced pulmonary hypertension