PXD046672-1

PXD046672 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Mason-Pfizer monkey virus virions
Description	To accomplish their replication cycle, retroviruses rely heavily on a wide variety of cellular proteins and enzymes, such as helicases. Among these, the DExD-box and DEAH/RHA-box helicases were identified to play crucial roles in their nucleic acid metabolism. This study explores the Betaretrovirus Mason-Pfizer monkey virus (M-PMV), which uniquely encodes a G-patch motif - a feature shared with several endogenous retroviruses. Typically found in eukaryotic RNA-binding proteins, G-patch acts as cofactors for DEAH-box RNA helicases, guiding them to specific sub-cellular sites and activating them in diverse RNA metabolic pathways. Despite its established importance in the betaretrovirus replication cycle, the function of the G-patch still needs to be discovered. Using MS analysis, we identified DHX15, a cellular DEAH-box RNA helicase, as host cell protein macked into mature virions. Further research illuminates the complex interplay between retroviruses and host cellular machinery, highlighting the pivotal role of helicases in viral replication processes.
HostingRepository	PRIDE
AnnounceDate	2024-09-13
AnnouncementXML	Submission_2024-09-13_02:34:42.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Junková
SpeciesList	scientific name: Mason-Pfizer monkey virus; NCBI TaxID: 11855;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-05 01:12:09	ID requested
⏵ 1	2024-09-13 02:34:43	announced

Dataset with its publication pending

submitter keyword: Mason-Pfizer monkey virus, DHX15, G-patch domain, HEK 293, LC-MS/MS

Michaela Rumlová
contact affiliation	Department of Biotechnology, University of Chemistry and Technology, Prague, 166 28 Prague, Czech Republic
contact email	rumlovai@vscht.cz
lab head
Petra Junková
contact affiliation	Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague
contact email	petra.junkova@uochb.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD046672

PRIDE project URI

• PRIDE
  1. PXD046672
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Mason-Pfizer monkey virus virions