PXD046632 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular insights into RNA polymerase recycling by mycobacterial HelD |
Description | Mycobacterial HelD is a transcription factor that binds RNA polymerase (RNAP) and thereby rescues it from stalled transcription complexes. HelD also protects RNAP against the antibiotic rifampicin. HelD, however, must be released from the rescued RNAP to participate in the next round of the transcription cycle. The exact mechanism of this release is unknown. Here we show that HelD from Mycobacterium smegmatis can form a complex with RNAP, together with σA primary sigma factor, and RbpA, but not CarD, transcription factors. Using cryo-EM, we solved a series of RNAP-σA-RbpA-HelD structures with or without promoter DNA. These snapshots capture the involvement of HelD in transcription initiation, describes mechanistic aspects of HelD release, and its protective effect against rifampicin. Biochemical evidence supports these findings, defines the role of ATP binding/hydrolysis to/by HelD in the process, and functionally confirms the rifampicin-protective effect of HelD. Taken together, HelD assists an alternative pathway of transcription initiation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_03:41:55.258.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD046632 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alena Krenkova |
SpeciesList | scientific name: Mycobacterium smegmatis; NCBI TaxID: 1772; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-03 03:05:12 | ID requested | |
⏵ 1 | 2024-10-08 03:41:55 | announced | |
2 | 2024-10-22 07:00:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: HelD |
RNA polymerase |
Mycobacteria |
Transcription |
Rifampicin |
Contact List
Martin Hubalek |
contact affiliation | IOCB CAS CR |
contact email | hubalek@uochb.cas.cz |
lab head | |
Alena Krenkova |
contact affiliation | IOCB CAS CR |
contact email | alena.krenkova@uochb.cas.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046632
- Label: PRIDE project
- Name: Molecular insights into RNA polymerase recycling by mycobacterial HelD