PXD046606-1

PXD046606 is an original dataset announced via ProteomeXchange.

Title	Understanding the interaction of SurA and BAM in the outer membrane of E. coli
Description	The outer membrane (OM) is a formidable barrier that protects Gram-negative bacteria against environmental threats. The correct folding and insertion of outer membrane proteins (OMPs) into the OM requires the essential OM-embedded β-barrel assembly machinery (BAM), a heptameric protein complex in E. coli. OMPs are delivered to BAM by the periplasmic chaperone SurA. However, how the activity of SurA and BAM are coordinated to ensure successful OMP delivery to BAM for folding into the OM remained unclear. We have trapped SurA in the act of OMP delivery to BAM and, via cryoEM, solved the structures of this assembly. By mutating key interaction sites we validate this interaction and use proteomics to determine how this perturbs the proteome of E. coli.
HostingRepository	PRIDE
AnnounceDate	2024-10-01
AnnouncementXML	Submission_2024-10-01_08:38:29.408.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Calabrese
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-02 04:07:03	ID requested
⏵ 1	2024-10-01 08:38:30	announced
2	2024-10-22 07:00:18	announced	2024-10-22: Updated project metadata.

Fenn KL, Horne JE, Crossley JA, B, ö, hringer N, Horne RJ, Sch, ä, berle TF, Calabrese AN, Radford SE, Ranson NA, Outer membrane protein assembly mediated by BAM-SurA complexes. Nat Commun, 15(1):7612(2024) [pubmed]

10.1038/s41467-024-51358-x;

submitter keyword: BAM,E coli, SurA, outer membrane

Antonio Calabrese
contact affiliation	Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, United Kingdom
contact email	a.calabrese@leeds.ac.uk
lab head
Antonio Calabrese
contact affiliation	School of Molecular and Cellular Biology, University of Leeds
contact email	a.calabrese@leeds.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD046606

PRIDE project URI

• PRIDE
  1. PXD046606
     1. Label: PRIDE project
     2. Name: Understanding the interaction of SurA and BAM in the outer membrane of E. coli