PXD046598-1

PXD046598 is an original dataset announced via ProteomeXchange.

Title	Assessment of Kinome-wide Rewiring Upon Picornavirus Infection
Description	Picornaviridae is a large family of single-stranded positive RNA viruses that can infect both humans and animals. These include the enteroviruses (e.g. poliovirus, coxsackievirus and rhinoviruses) as well as the cardioviruses (e.g. encephalomyocarditis virus (EMCV)). Picornaviruses have evolved to interact, use and/or evade cellular host systems to create the optimal environment for viral growth and spreading. It is known that these viruses can modify kinase activity during infection, but a comprehensive overview of the (de)regulation of cellular kinases during picornavirus infection is lacking. To study the landscape of kinase activity during picornavirus infection, we here applied dedicated targeted mass spectrometry-based assays targeting ~40% of the human kinome. Our data show that kinases of the MAPK pathways were activated (e.g. ERK1/2, RSK1/2, JNK1/2/3, p38), while kinase involved in regulating the cell cycle (e.g. CDK1/2, GWL, DYRK3) were inactivated. Additionally, we observed the activation of CHK2, an important kinase of the DNA damage response. Using pharmacological kinases inhibitors, we demonstrate that some of the activated kinases are essential for the replication of EMCV. Altogether, we provide a quantitative system-wide understanding of the regulation of kinome activity induced by picornavirus infection, which is important for developing novel therapeutic interventions.
HostingRepository	PanoramaPublic
AnnounceDate	2024-04-21
AnnouncementXML	Submission_2024-04-21_22:38:27.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Tim Veth
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Phospho; Carbamidomethyl; Carboxymethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-01 19:02:02	ID requested
⏵ 1	2024-04-21 22:38:28	announced

Veth TS, Nouwen LV, Zwaagstra M, Lyoo H, Wierenga KA, Westendorp B, Altelaar MAFM, Berkers C, van Kuppeveld FJM, Heck AJR, Assessment of Kinome-Wide Activity Remodeling upon Picornavirus Infection. Mol Cell Proteomics, 23(5):100757(2024) [pubmed]

submitter keyword: Picornavirus, kinome, phosphorylation, kinase activation loop, phosphoproteomics, CVB3, EMCV, 2A, L protein, Viral life cycle

Albert Heck
contact affiliation	Utrecht University
contact email	a.j.r.heck@uu.nl
lab head
Tim Veth
contact affiliation	Utrecht University
contact email	t.s.veth@uu.nl
dataset submitter

Panorama Public dataset URI