<<< Full experiment listing


PXD046598 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAssessment of Kinome-wide Rewiring Upon Picornavirus Infection
DescriptionPicornaviridae is a large family of single-stranded positive RNA viruses that can infect both humans and animals. These include the enteroviruses (e.g. poliovirus, coxsackievirus and rhinoviruses) as well as the cardioviruses (e.g. encephalomyocarditis virus (EMCV)). Picornaviruses have evolved to interact, use and/or evade cellular host systems to create the optimal environment for viral growth and spreading. It is known that these viruses can modify kinase activity during infection, but a comprehensive overview of the (de)regulation of cellular kinases during picornavirus infection is lacking. To study the landscape of kinase activity during picornavirus infection, we here applied dedicated targeted mass spectrometry-based assays targeting ~40% of the human kinome. Our data show that kinases of the MAPK pathways were activated (e.g. ERK1/2, RSK1/2, JNK1/2/3, p38), while kinase involved in regulating the cell cycle (e.g. CDK1/2, GWL, DYRK3) were inactivated. Additionally, we observed the activation of CHK2, an important kinase of the DNA damage response. Using pharmacological kinases inhibitors, we demonstrate that some of the activated kinases are essential for the replication of EMCV. Altogether, we provide a quantitative system-wide understanding of the regulation of kinome activity induced by picornavirus infection, which is important for developing novel therapeutic interventions.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterTim Veth
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListPhospho; Carbamidomethyl; Carboxymethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentTSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-01 19:02:02ID requested
12024-04-21 22:38:28announced
Publication List
Veth TS, Nouwen LV, Zwaagstra M, Lyoo H, Wierenga KA, Westendorp B, Altelaar MAFM, Berkers C, van Kuppeveld FJM, Heck AJR, Assessment of Kinome-Wide Activity Remodeling upon Picornavirus Infection. Mol Cell Proteomics, 23(5):100757(2024) [pubmed]
Keyword List
submitter keyword: Picornavirus, kinome, phosphorylation, kinase activation loop, phosphoproteomics, CVB3, EMCV, 2A, L protein, Viral life cycle
Contact List
Albert Heck
contact affiliationUtrecht University
contact emaila.j.r.heck@uu.nl
lab head
Tim Veth
contact affiliationUtrecht University
contact emailt.s.veth@uu.nl
dataset submitter
Full Dataset Link List
Panorama Public dataset URI