PXD046570-1

PXD046570 is an original dataset announced via ProteomeXchange.

Title	Caspase-4 and Caspase-5 Coordinate the Sensing of Streptococcus pyogenes Extracellular vesicles
Description	Caspase-4 and caspase-5 have been linked to the sensing of Gram-negative bacteria and their extracellular vesicles (EVs). Recent studies suggested that Gram-positive bacteria can be sensed through signaling cascades involving these two caspases. In the present study, we report that S. pyogenes secretes vesicles decorated with LTA on their surface that can activate caspase-4/-5 and the NLRP3 inflammasome. We show that this sensing process depends on particle internalization and Toll-like receptor (TLR) signaling. Finally, we demonstrate that caspase-4/-5 participate in the recognition of EVs from other Gram-positive bacteria.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_17:05:01.856.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-01 04:56:45	ID requested
⏵ 1	2025-10-19 17:05:02	announced

Krause K, Franch Arroyo S, Ugolini M, Kueck T, Sullivan TJ, G, á, lvez EJC, Muenzner M, Goosmann C, Brinkmann V, Frese CK, Alagesan K, Vierbuchen T, Heine H, Resch U, Sander LE, Charpentier E, Streptococcus pyogenes EVs induce the alternative inflammasome via caspase-4/-5 in human monocytes. EMBO Rep, 26(19):4847-4885(2025) [pubmed]

10.1038/s44319-025-00558-7;

submitter keyword: S. pyogenes, NLRP3, caspases

Emmanuelle Charpentier
contact affiliation	Max Planck Unit for the Science of Pathogens, D-10117 Berlin, Germany Institute for Biology, Humboldt University, D-10115 Berlin, Germany
contact email	research-charpentier@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD046570

PRIDE project URI

• PRIDE
  1. PXD046570
     1. Label: PRIDE project
     2. Name: Caspase-4 and Caspase-5 Coordinate the Sensing of Streptococcus pyogenes Extracellular vesicles