PXD046546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RNA polymerase II acts to promote the organization of chromatin following DNA replication |
Description | In mammals, following the passage of the replisome, nucleosomes lose their defined positioning and transcription contributes to their re-organisation. However, whether transcription plays a greater role in the organization of chromatin following DNA replication remains unclear. By performing iPOND-TMT time course experiments in the presents of transcription inhibition, we show that RNAPII promotes the re-association of hundreds of proteins with newly replicated DNA (Data uploaded under PXD046514). To show that this function of RNAPII is specific to newly replicated chromatin, an iPOND-TMT 24hr chase experiment was performed in the presence of transcription inhibition to examine its effect on the protein binding on steady state chromatin. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-14 |
AnnouncementXML | Submission_2024-02-14_07:53:29.052.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Susanne Bandau |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-31 13:33:53 | ID requested | |
⏵ 1 | 2024-02-14 07:53:29 | announced | |
Publication List
10.1038/s44319-024-00085-x; |
Bandau S, Alvarez V, Jiang H, Graff S, Sundaramoorthy R, Gierlinski M, Toman M, Owen-Hughes T, Sidoli S, Lamond A, Alabert C, RNA polymerase II promotes the organization of chromatin following DNA replication. EMBO Rep, 25(3):1387-1414(2024) [pubmed] |
Keyword List
submitter keyword: TMT,Proteomics, DNA replications |
Contact List
Constance Alabert |
contact affiliation | Molecular Cell and Developmental Biology, School of Life Sciences, University of Dundee, United Kingdom |
contact email | c.alabert@dundee.ac.uk |
lab head | |
Susanne Bandau |
contact affiliation | University of Dundee |
contact email | sbandau@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046546 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046546
- Label: PRIDE project
- Name: RNA polymerase II acts to promote the organization of chromatin following DNA replication