PXD046528-1

PXD046528 is an original dataset announced via ProteomeXchange.

Title	The generation of cancer-specific ribosomes by the lncRNA LISR suppresses melanoma anti-tumour immunity
Description	Gains of chromosome 12p11.21, encoding for the cancer-specific lncRNA LISR, correlate with poor survival in the PanCancer Atlas. In melanoma, LISR is highly expressed by patients resistant to immunotherapy and it contributes to the generation of drug-tolerant persister cells by activating an immune suppressive translational program affecting the synthesis of PD-L1 and of the glycocalyx. Interestingly, the use of glycans to evade the immune system is a characteristic of the sperm. Accordingly, a defective glycocalyx assembly during spermatogenesis causes infertility. Here we show that LISR affects the core ribosome composition and recruits Deleted in AZoospermia Associated Protein 1 (DAZAP1) to regulate an immune suppressive translational program that includes PD-L1 and several glycosylases. Consequently, down-regulation of LISR leads to robust anti-tumour immune responses and re-sensitizes to immune checkpoint blockade. Our study reveals the contribution of lncRNAs to the generation of cancer-specific ribosomes and identifies an RNA-based cancer-specific strategy to overcome intrinsic resistance to immune checkpoint +blockade.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_10:07:47.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	alessandro cuomo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-31 04:30:28	ID requested
⏵ 1	2025-09-17 10:07:48	announced

10.1084/JEM.20251507;

submitter keyword: Ribosomes,Long non coding RNA

Eleonora Leucci
contact affiliation	Laboratory for RNA Cancer Biology Department of Oncology, LKI, KU Leuven
contact email	eleonora.leucci@kuleuven.be
lab head
alessandro cuomo
contact affiliation	IEO
contact email	alessandro.cuomo@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD046528

PRIDE project URI

• PRIDE
  1. PXD046528
     1. Label: PRIDE project
     2. Name: The generation of cancer-specific ribosomes by the lncRNA LISR suppresses melanoma anti-tumour immunity