PXD046477-3

PXD046477 is an original dataset announced via ProteomeXchange.

Title	Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
Description	This study aimed to reveal the influence of co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus on the proteome of C. jejuni 81–176 using data-independent-acquisition mass spectrometry (DIA-MS). We compared the proteome profiles during co-incubation with the proteome profile in response to the bile acid deoxycholate (DCA) and investigated the impact of DCA on proteomic changes during co-incubation, as C. jejuni is exposed to both factors during colonization. We identified 1,375 proteins by DIA-MS, which is notably high, approaching the theoretical maximum of 1,645 proteins. S. aureus had the highest impact on the proteome of C. jejuni with 215 up-regulated and 230 down-regulated proteins. However, these numbers are still markedly lower than the 526 up-regulated and 516 down-regulated proteins during DCA exposure.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:40.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni 81-176; NCBI TaxID: 354242;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-29 11:57:09	ID requested
1	2023-11-02 08:51:24	announced
2	2023-11-14 09:09:32	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:40	announced	2024-10-22: Updated project metadata.

10.3389/FMICB.2023.1247211;

submitter keyword: co-incubation, proteomics, Staphylococcus aureus, Enterococcus faecalis, bile acids,Campylobacter jejuni, Enterococcus faecium

Christof Lenz
contact affiliation	University Medical Center Goettingen, Department of Clinical Chemistry, Core Facility Proteomics
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046477

PRIDE project URI

• PRIDE
  1. PXD046477
     1. Label: PRIDE project
     2. Name: Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios