PXD046472-1

PXD046472 is an original dataset announced via ProteomeXchange.

Title	The SETD3 interacting protein was identified and screened by TurboID method, and Search for potential histidine methylation modification sites
Description	we employed a TurboID approach that utilized a modified promiscuous biotin ligase BirA for proximity labeling by fusing SETD3 with this enzyme, By introducing biotin, cellular fractions of SETD3-TurboID-transfected cells, both cytosolic and chromatin fractions, underwent immunoprecipitation, followed by MS analysis to identify SETD3-interacting proteins. We conducted two experiments, first time we tested four samples, the RAW data of cyto_Vec and chr_Vec represented cells experssing emptry vector soluble fraction and chromatin fraction respectively. the RAW data of cyto_SET and chr_SET represented cells experssing SETD3-TurboID soluble fraction and chromatin fraction respectively. And PSMs.xlsx described the analysis of mass spectrum data. Second time we tested three samples. The RAW data of 21010704_DHG_VEC, 21010704_DHG_WT and 21010704_DHG_YA represented cells experssing emptry vector, SETD3-WT-TurboID or SETD3-Y313A-TurboID respectively. 21010704_DHG_Protein-compare.xlsx described the proteins identification of mass spectrum data. 21010704_DHG_Methyl-H.xlsx listed the histidine methylation identification of mass spectrum data.
HostingRepository	PRIDE
AnnounceDate	2024-03-12
AnnouncementXML	Submission_2024-03-12_02:20:40.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	D HG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-29 00:41:06	ID requested
⏵ 1	2024-03-12 02:20:41	announced

Dataset with its publication pending

submitter keyword: TurboID,HeLa S3, LC-MS/MS

Hai-Ning Du
contact affiliation	Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Frontier Science Center for Immunology and Metabolism, Emergency Center, Zhongnan Hospital of Wuhan University, RNA Institute, Wuhan University
contact email	hainingdu@whu.edu.cn
lab head
D HG
contact affiliation	Wuhan University
contact email	dhg@whu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046472
     1. Label: PRIDE project
     2. Name: The SETD3 interacting protein was identified and screened by TurboID method, and Search for potential histidine methylation modification sites