PXD046439-2

PXD046439 is an original dataset announced via ProteomeXchange.

Title	Aspergillus fumigatus interaction with Klebsiella pneumoniae in biofilm mode
Description	This project aimed to characterize inter-species interactions between the A. fumigatus and K. pneumoniae microbial pathogens by analyzing the proteomic profiles of single-pathogen cultures and co-cultures. Biofilms of both A. fumigatus and K. pneumoniae were grown for 24 hours at 37°C in 3 mL of YNBP minimal medium [1.7 g/L yeast nitrogen base (YNB) without amino acids, 5g/L ammonium sulfate, 25 mM phosphate buffer pH 7.0 (KH2PO4 + K2HPO4), 2.5 mM N-acetyl-glucosamine, 0.2% glucose, 0.1% maltose]. Protein pellets obtained from these conditions were further analyzed, revealing metabolic rearrangements and differences in cell cycle regulation due to the interaction. Proteomic profiling, in general, provided new insights into the metabolic reshaping critical for the pathogens to navigate in the course of polymicrobial infection. Additionally, an exploration of the produced compounds uncovered potential biomarker candidates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:39.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 23:53:29	ID requested
1	2024-10-03 07:53:44	announced
⏵ 2	2024-10-22 07:00:40	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: biofilm, microbial interaction, bacteria, biomarkers, fungi

Thomas Lion
contact affiliation	CCRI – St. Anna Children's Cancer Research Institute, Vienna, Austria Labdia - Labordiagnostik GmbH, Vienna, Austria Department of Pediatrics, Medical University of Vienna, Austria
contact email	thomas.lion@ccri.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD046439

PRIDE project URI

• PRIDE
  1. PXD046439
     1. Label: PRIDE project
     2. Name: Aspergillus fumigatus interaction with Klebsiella pneumoniae in biofilm mode