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PXD046439-2

PXD046439 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAspergillus fumigatus interaction with Klebsiella pneumoniae in biofilm mode
DescriptionThis project aimed to characterize inter-species interactions between the A. fumigatus and K. pneumoniae microbial pathogens by analyzing the proteomic profiles of single-pathogen cultures and co-cultures. Biofilms of both A. fumigatus and K. pneumoniae were grown for 24 hours at 37°C in 3 mL of YNBP minimal medium [1.7 g/L yeast nitrogen base (YNB) without amino acids, 5g/L ammonium sulfate, 25 mM phosphate buffer pH 7.0 (KH2PO4 + K2HPO4), 2.5 mM N-acetyl-glucosamine, 0.2% glucose, 0.1% maltose]. Protein pellets obtained from these conditions were further analyzed, revealing metabolic rearrangements and differences in cell cycle regulation due to the interaction. Proteomic profiling, in general, provided new insights into the metabolic reshaping critical for the pathogens to navigate in the course of polymicrobial infection. Additionally, an exploration of the produced compounds uncovered potential biomarker candidates.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_07:00:39.659.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWeiQiang Chen
SpeciesList scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-26 23:53:29ID requested
12024-10-03 07:53:44announced
22024-10-22 07:00:40announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: biofilm, microbial interaction, bacteria, biomarkers, fungi
Contact List
Thomas Lion
contact affiliationCCRI – St. Anna Children's Cancer Research Institute, Vienna, Austria Labdia - Labordiagnostik GmbH, Vienna, Austria Department of Pediatrics, Medical University of Vienna, Austria
contact emailthomas.lion@ccri.at
lab head
WeiQiang Chen
contact affiliationMass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact emailchenweiqiang@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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