PXD046435-1

PXD046435 is an original dataset announced via ProteomeXchange.

Title	Dimethylthiourea as a Quencher in Hydroxyl Radical Protein Footprinting Experiments
Description	Hydroxyl radical protein footprinting (HRPF) is a mass spectrometry (MS)-based method for studying protein structures, interactions, conformations, and folding. We evaluated dimethylthiourea (DMTU) as a replacement for catalase in quenching HRPF reactions. We observed that the DMTU is highly effective at quenching HRPF oxidation. DMTU does not cause background protein issues like catalase, resulting in an increased number of protein identifications from complex mixtures.
HostingRepository	PRIDE
AnnounceDate	2025-09-12
AnnouncementXML	Submission_2025-09-12_13:50:45.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandeep Misra
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 19:31:50	ID requested
⏵ 1	2025-09-12 13:50:45	announced

10.1021/jasms.3c00323;

Shami AA, Misra SK, Jones LM, Sharp JS, Dimethylthiourea as a Quencher in Hydroxyl Radical Protein Footprinting Experiments. J Am Soc Mass Spectrom, 34(12):2864-2867(2023) [pubmed]

submitter keyword: HRPF,Dimethylthiourea, quencher

Joshua Sharp
contact affiliation	Depatment of Biomolecular Sciences University of Mississippi
contact email	skmisra@olemiss.edu
lab head
Sandeep Misra
contact affiliation	University of Mississippi
contact email	skmisra@olemiss.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD046435

PRIDE project URI

• PRIDE
  1. PXD046435
     1. Label: PRIDE project
     2. Name: Dimethylthiourea as a Quencher in Hydroxyl Radical Protein Footprinting Experiments