PXD046423-1

PXD046423 is an original dataset announced via ProteomeXchange.

Title	Epitope mapping of SARS-CoV 2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls.
Description	The focus of the present investigation was epitope mapping of a mouse monoclonal antibody to the receptor binding domain of two SARS-CoV-2 spike protein variants (Wild-Type and variant B.1.617.2). The experimental setup utilizing multiple controls allowed for validation of false positive binding on several levels, while still being easily implementable in the general MS laboratory. The primary equipment needed to implement this method in addition to a mass spectrometer is a pipetting robot e.g., Opentrons OT-2.
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_05:58:31.922.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Nyberg Larsen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; dihydroxylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 08:39:25	ID requested
⏵ 1	2025-02-21 05:58:32	announced

10.1016/J.BBAPAP.2024.141011;

submitter keyword: RBD Alpha, Fenton chemistry, Covid-19, Mass spectrometry, RBD Delta

Peter Højrup
contact affiliation	University of Southern Denmark, Department of Biochemistry and Molecular Biology, Protein Research
contact email	php@bmb.sdu.dk
lab head
Daniel Nyberg Larsen
contact affiliation	University of Southern Denmark
contact email	daniel.nyberg@live.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD046423

PRIDE project URI

• PRIDE
  1. PXD046423
     1. Label: PRIDE project
     2. Name: Epitope mapping of SARS-CoV 2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls.