PXD046415-1

PXD046415 is an original dataset announced via ProteomeXchange.

Title	MS analysis of STING linear ubiquitinated sites
Description	HEK293T cells transiently transfected with plasmids encoding Flag-STING, Myc-HOIP and HA-HOIL-1L, were lysed with buffer (20 mM Na2HPO4, 20 mM NaH2PO4, 1% NP-40, 2 mM EDTA) supplemented with 1 mM DTT, 5 mM NEM, complete protease inhibitor cocktail, PhosSTOP and 0.1% SDS. The lysates were incubated with Anti-Flag M2 Affinity Gel. The bound material was eluted with 0.1 M glycine-HCl pH 2.7, separated by electrophoresis in a 10% gel, and stained with Bio-Safe Coomassie and then for mass spectrometry analysis
HostingRepository	iProX
AnnounceDate	2023-10-26
AnnouncementXML	Submission_2024-10-10_19:10:13.312.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yesheng Fu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 05:44:19	ID requested
⏵ 1	2024-10-10 19:10:13	announced

Dataset with its publication pending

submitter keyword: STING, linear ubiquitination

Lingqiang Zhang
contact affiliation	Beijing Institute of Lifeomics
contact email	zhanglq@nic.bmi.ac.cn
lab head
Yesheng Fu
contact affiliation	Beijing Institute of Lifeomics
contact email	fyshwork2013@126.com
dataset submitter

iProX dataset URI