PXD046415-1
PXD046415 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | MS analysis of STING linear ubiquitinated sites |
Description | HEK293T cells transiently transfected with plasmids encoding Flag-STING, Myc-HOIP and HA-HOIL-1L, were lysed with buffer (20 mM Na2HPO4, 20 mM NaH2PO4, 1% NP-40, 2 mM EDTA) supplemented with 1 mM DTT, 5 mM NEM, complete protease inhibitor cocktail, PhosSTOP and 0.1% SDS. The lysates were incubated with Anti-Flag M2 Affinity Gel. The bound material was eluted with 0.1 M glycine-HCl pH 2.7, separated by electrophoresis in a 10% gel, and stained with Bio-Safe Coomassie and then for mass spectrometry analysis |
HostingRepository | iProX |
AnnounceDate | 2023-10-26 |
AnnouncementXML | Submission_2024-10-10_19:10:13.312.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yesheng Fu |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | ubiquitination signature dipeptidyl lysine |
Instrument | 4800 Proteomics Analyzer |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-26 05:44:19 | ID requested | |
⏵ 1 | 2024-10-10 19:10:13 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: STING, linear ubiquitination |
Contact List
Lingqiang Zhang | |
---|---|
contact affiliation | Beijing Institute of Lifeomics |
contact email | zhanglq@nic.bmi.ac.cn |
lab head | |
Yesheng Fu | |
contact affiliation | Beijing Institute of Lifeomics |
contact email | fyshwork2013@126.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |