PXD046413-2

PXD046413 is an original dataset announced via ProteomeXchange.

Title	Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics
Description	Protein-protein interactions play an important biological role in every aspect of cellular homeostasis and functioning. Proximity labeling mass spectrometry-based proteomics overcomes challenges typically associated with other methods, and has quickly become the current state-of-the-art in the field. Nevertheless, tight control of proximity labeling enzymatic activity and expression levels is crucial to accurately identify protein interactors. Here, we leverage a T2A self-cleaving peptide and a non-cleaving mutant to allow the use of the protein-of-interest in the experimental and control TurboID setup. To allow easy and streamlined plasmid assembly, we build a Golden Gate modular cloning system to generate plasmids for transient expression and stable integration. To highlight our T2A Split-link design, we applied it to identify protein interactions of the glucocorticoid receptor and SARS-CoV-2 nucleocapsid and NSP7 proteins by TurboID proximity labeling. Our results demonstrate that our T2A split/link provides an opportune control to match expression levels for proximity labeling proteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:44:10.615.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louis Delhaye
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 04:58:59	ID requested
1	2024-06-10 01:56:53	announced
⏵ 2	2024-10-22 06:44:10	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: BioID, SARS-CoV-2 NSP7, TurboID, proximity labeling, GR, SARS-CoV-2 N

Sven Eyckerman
contact affiliation	VIB-UGent Center for Medical Biotechnology
contact email	sven.eyckerman@ugent.vib.be
lab head
Louis Delhaye
contact affiliation	VIB-UGent
contact email	louis.delhaye@ugent.vib.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD046413

PRIDE project URI

• PRIDE
  1. PXD046413
     1. Label: PRIDE project
     2. Name: Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics