PXD046411-1

PXD046411 is an original dataset announced via ProteomeXchange.

Title	Mapping the immunopeptidome of several SARS-CoV-2 antigens across common HLA haplotypes
Description	Most COVID-19 vaccines have been designed to elicit immunity against the SARS-CoV-2 Spike protein. However, the repeated occurrence of new strains harbouring Spike protein mutations demonstrates ready immune evasion by the SARS-CoV-2 virus and the pressing need to develop more broadly targeting COVID-19 vaccines. To facilitate this, we used mass spectrometry to identify immunopeptides that are derived from seven structural and non-structural SARS-CoV-2 proteins that are relatively conserved across viral strains (N, E, Nsp1, Nsp4, Nsp5, Nsp8, Nsp9) and presented by prevalent Human Leukocyte Antigen (HLA) class I and class II molecules. Two different B-lymphoblastoid cell lines were chosen to map immunopeptidomes covering some of the major HLA types across the global human population. We used DNA plasmid transfection and direct antigen delivery approaches to sample different antigens. We found 248 unique HLA class I and HLA class II bound peptides with 12 derived from E, 71 from N, 28 from Nsp1, 19 from Nsp4, 73 from Nsp8 and 45 peptides derived from Nsp9. T cell responses were tested for 56 of the detected peptides and we show robust CD8+ and CD4+ T cell responses against several peptides from the N, E and Nsp9 proteins. Results from this study will aid the development of next-generation COVID vaccines targeting epitopes from across a number of SARS-CoV-2 proteins.
HostingRepository	MassIVE
AnnounceDate	2024-08-03
AnnouncementXML	Submission_2024-08-03_17:46:34.963.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	A. Braun
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	Oxidation; Acetyl; Deamidated
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 03:34:56	ID requested
⏵ 1	2024-08-03 17:46:35	announced

no publication

submitter keyword: B lymphoblastoid cell line, SARS-CoV-2, COVID-19

Anthony Wayne Purcell
contact affiliation	Deputy Head (Research), Department of Biochemistry and Molecular Biology Head Immunoproteomics Laboratory Infection and Immunity Program Monash Biomedicine Discovery Institute Monash University, Clayton Campus Clayton 3800 Victoria Australia
contact email	anthony.purcell@monash.edu
lab head
A. Braun
contact affiliation	Monash
contact email	assolbraun@web.de
dataset submitter

MassIVE dataset URI

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