PXD046386-2

PXD046386 is an original dataset announced via ProteomeXchange.

Title	Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition. Yeast KO collection
Description	Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes in a fast and reproducible manner. Here, we present an ultra-fast scanning data-independent acquisition (DIA) strategy consisting on 2-Th precursor isolation windows, dissolving the differences between data-dependent and independent methods. This is achieved by pairing a Quadrupole Orbitrap mass spectrometer with the asymmetric track lossless (Astral) analyzer that provides >200 Hz MS/MS scanning speed, high resolving power and sensitivity, as well as low ppm-mass accuracy. Narrowwindow DIA enables profiling of up to 100 full yeast proteomes per day, or ~10,000 human proteins in half-an-hour. Moreover, multi-shot acquisition of fractionated samples allows comprehensive coverage of human proteomes in ~3h, showing comparable depth to next-generation RNA sequencing and with 10x higher throughput compared to current state-of-the-art MS. High quantitative precision and accuracy is demonstrated with high peptide coverage in a 3-species proteome mixture, quantifying 14,000+ proteins in a single run in half-an-hour.
HostingRepository	PRIDE
AnnounceDate	2024-02-13
AnnouncementXML	Submission_2024-02-13_03:26:33.580.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulises H Guzman
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-25 07:11:16	ID requested
1	2024-02-12 09:03:45	announced
⏵ 2	2024-02-13 03:26:33	announced	2024-02-13: Updated project metadata.

10.1038/S41587-023-02099-7;

submitter keyword: DIA, Astral,Proteomics

Jesper V.
contact affiliation	Professor, Deputy Head of Centre Novo Nordisk Foundation Center for Protein Research University of Copenhagen Copenhagen, DENMARK
contact email	jesper.olsen@cpr.ku.dk
lab head
Ulises H Guzman
contact affiliation	Novo Nordisk Foundation Center for Protein Research
contact email	ulysseshrdz@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046386

PRIDE project URI

• PRIDE
  1. PXD046386
     1. Label: PRIDE project
     2. Name: Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition. Yeast KO collection