PXD046385 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ribosomal Protein SA (RPSA) is required for localized translation and sarcomere maintenance |
Description | Cardiomyocyte sarcomeres contain localized ribosomes, but the factors responsible for their localization and the significance of localized translation are unknown. Using proximity labeling, we identified Ribosomal Protein SA (RPSA) as a Z-line protein. In cultured cardiomyocytes, the loss of RPSA led to impaired local protein translation and reduced sarcomere integrity. By employing CAS9 expression mice along with adeno-associated viruses expressing CRE recombinase and single-guide RNAs targeting Rpsa, we knocked out RPSA in vivo and observed mis-localization of ribosomes and diminished local translation. These genetic mosaic mice with RPSA knockout in a subset of cardiomyocytes developed dilated cardiomyopathy, featuring atrophy of RPSA-deficient cardiomyocytes, compensatory hypertrophy of unaffected cardiomyocytes, left ventricular dilation, and impaired contractile function. We demonstrate that RPSA C-terminal domain is sufficient for localization Z-lines. These findings highlight RPSA as a ribosomal factor responsible for ribosome localization to the Z-line, facilitating local translation and sarcomere maintenance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-04 |
AnnouncementXML | Submission_2024-06-04_09:52:11.754.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamar Ziv |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-25 06:50:21 | ID requested | |
⏵ 1 | 2024-06-04 09:52:12 | announced | |
2 | 2024-10-22 06:43:46 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ribosome, Localized translation, proximity labeling, Sarcomere, Z-line |
Contact List
Izhak Kehat |
contact affiliation | 2The Rappaport Institute and the Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Israel. |
contact email | ikehat@technion.ac.il |
lab head | |
Tamar Ziv |
contact affiliation | Technion |
contact email | tamarz@technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD046385 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046385
- Label: PRIDE project
- Name: Ribosomal Protein SA (RPSA) is required for localized translation and sarcomere maintenance