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PXD046374-1

PXD046374 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The phosphorylation sites in GLI1 analyzed by mass-spec methodology
Description	The mass spectrometry analysis was performed by Micrometer Biotech Company. Endogenous human GLI1 from Daoy cells was enriched by co-immunoprecipitation with anti-Gli1 and purified by SDS-PAGE. The gel was then stained with Coomassie brilliant blue, and the corresponding visualized bands were excised, destained with ammonium bicarbonate buffer and dehydrated in 75% acetonitrile. After rehydration with ammonium bicarbonate, trypsin digestion was performed, followed by peptide extraction with acetonitrile, drying, and mass spectrometry
HostingRepository	iProX
AnnounceDate	2023-10-25
AnnouncementXML	Submission_2023-10-24_23:01:35.580.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jirong Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-24 23:01:18	ID requested
⏵ 1	2023-10-24 23:01:35	announced
2	2024-02-26 20:07:38	announced	2024-02-27: Update information.

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: phosphorylation sites, GLI1

Contact List

Ximei Wu
contact affiliation	Zhejiang University
contact email	xiwu@zju.edu.cn
lab head
Jirong Wang
contact affiliation	Zhejiang University
contact email	wangjr@zju.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI