PXD046332-2

PXD046332 is an original dataset announced via ProteomeXchange.

Title	Identification and Characterization of a Pepsin and Chymotrypsin-Resistant Peptide in the α Subunit of the 11S Globulin Legumin from Common Bean (Phaseolus vulgaris L.)
Description	The 11S globulin legumin typically accounts for approximately 3% of total protein in common bean (Phaseolus vulgaris). It was previously reported that a legumin peptide of approximately 20 kDa is resistant to pepsin digestion. Sequence prediction suggested that the pepsin resistant peptide is located at the C-terminal end of the α-subunit, within a glutamic acid-rich domain, overlapping with a chymotrypsin-resistant peptide. Using purified legumin, the peptide of ca. 20 kDa was found to be resistant to pepsin digestion in a pH-dependent manner, and its location was determined by two-dimensional gel electrophoresis and LC-MS-MS. Sample 1 corresponds to trypsin spot. Sample 2 corresponds to legumin alpha subunit spot of 63 kDa. Sample 3 corresponds to legumin alpha subunit spot of 48 kDa. Sample 4 corresponds to legumin beta subunit spot. Sample 5 corresponds to pepsin resistant spot of alpha subunit.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:47.687.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046332
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frédéric Marsolais
SpeciesList	scientific name: Phaseolus vulgaris (Kidney bean) (French bean); NCBI TaxID: 3885;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 11:20:52	ID requested
1	2024-08-09 05:06:08	announced
⏵ 2	2024-10-22 06:53:51	announced	2024-10-22: Updated project metadata.

10.1021/acs.jafc.3c08744;

Santamaria L, Pajak A, House JD, Marsolais F, L.). J Agric Food Chem, 72(26):14844-14850(2024) [pubmed]

10.6019/PXD046332;

submitter keyword: resistance to proteolytic digestion, 11S globulin,legumin, pepsin, Phaseolus vulgaris, chymotrypsin, common bean

Frédéric Marsolais
contact affiliation	London Research and Development Centre Agriculture and Agri-Food Canada
contact email	Frederic.marsolais@agr.gc.ca
lab head
Frédéric Marsolais
contact affiliation	Agriculture and Agri-Food Canada
contact email	frederic.marsolais@agr.gc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD046332

PRIDE project URI

• PRIDE
  1. PXD046332
     1. Label: PRIDE project
     2. Name: Identification and Characterization of a Pepsin and Chymotrypsin-Resistant Peptide in the α Subunit of the 11S Globulin Legumin from Common Bean (Phaseolus vulgaris L.)