PXD046330-2

PXD046330 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SKBR3/HER2+ Breast Cancer Cellular Response to Brain Cell-Secreted Factors
Description	To address the challenges associated with the investigation of brain-metastasized cancers and provide insights into the drivers of cancer cell behavior at the brain metastatic site, this study was aimed at exploring the response of SKBR3/HER2+ cells to neural factors secreted by brain cells that come first in contact with the metastasized cancer cells, i.e., human endothelial cells (HBEC5i), astrocytes (NHA), and microglia (HMC3). To this end, serum-starved breast cancer cells were exposed to the combined secretome of brain cells, allowed to grow for 24h, and analyzed by LC-MS/MS using an Orbitrap QE instrument to generate and compare the whole cell proteome profiles of treated vs non-treated cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:45.851.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iulia M. Lazar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 10:01:04	ID requested
1	2024-08-27 07:19:18	announced
⏵ 2	2024-10-22 06:56:46	announced	2024-10-22: Updated project metadata.

Publication List

S41598-024-70386-7;

S41598-024-70386-7;

Keyword List

submitter keyword: HMC3, brain, SKBR3, NHA,Breast cancer, HBEC5i, metastasis

submitter keyword: HMC3, brain, SKBR3, NHA,Breast cancer, HBEC5i, metastasis

Contact List

Iulia M Lazar
contact affiliation	Virginia Tech
contact email	malazar@vt.edu
lab head
Iulia M. Lazar
contact affiliation	Virginia Tech
contact email	malazar@vt.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD046330
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD046330

PRIDE project URI

Repository Record List

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