PXD046315-1

PXD046315 is an original dataset announced via ProteomeXchange.

Title	Rauvolfia tetraphylla latex proteome
Description	Monoterpene indole alkaloids (MIAs) are a structurally diverse family of specialized metabolites mainly produced in Gentianales to cope with environmental challenges. Due to their pharmacological properties, the biosynthetic modalities of several MIA types have been elucidated but not that of the yohimbanes. Here, we combine transcriptomics and genome sequencing of Rauvolfia tetraphylla with machine learning to discover the unexpected multiple actors of this natural product's synthesis. We identify a medium chain dehydrogenase/reductase (MDR) that produces a mixture of four diastereomers of yohimbanes including the well-known yohimbine and rauwolscine. In addition to this multifunctional yohimbane synthase (YOS), an MDR synthesizing mainly heteroyohimbanes and the short chain dehydrogenase vitrosamine synthase also display a yohimbane synthase side activity. Lastly, we establish that the combination of geissoschizine synthase with at least three other MDRs also produces a yohimbane mixture thus shedding light on the complex mechanisms evolved for the synthesis of these plant bioactives.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:50:15.447.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clément Cuello
SpeciesList	scientific name: Rauvolfia tetraphylla; NCBI TaxID: 545371;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 01:47:07	ID requested
⏵ 1	2024-01-26 06:50:16	announced

10.1038/s42003-023-05574-8;

Stander EA, Lehka B, Carqueijeiro I, Cuello C, Hansson FG, Jansen HJ, Dug, é, De Bernonville T, Birer Williams C, Verg, è, s V, Lezin E, Lorensen MDBB, Dang TT, Oudin A, Lanoue A, Durand M, Giglioli-Guivarc'h N, Janfelt C, Papon N, Dirks RP, O'connor SE, Jensen MK, Besseau S, Courdavault V, The Rauvolfia tetraphylla genome suggests multiple distinct biosynthetic routes for yohimbane monoterpene indole alkaloids. Commun Biol, 6(1):1197(2023) [pubmed]

submitter keyword: yohimbane, medium chain dehydrogenase/reductase, rauwolscine, yohimbine,alkaloid, geissoschizine

Vincent Courdavault
contact affiliation	Biomolécules et Biotechnologies Végétales, EA2106, Université de Tours, 37200 Tours, France
contact email	vincent.courdavault@univ-tours.fr
lab head
Clément Cuello
contact affiliation	EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours
contact email	clement.cuello@univ-tours.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046315

PRIDE project URI

• PRIDE
  1. PXD046315
     1. Label: PRIDE project
     2. Name: Rauvolfia tetraphylla latex proteome