PXD046282-1
PXD046282 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cell cycle-dependent gene networks for cell proliferation activated by nuclear CK2 complexes |
| Description | Protein kinase CK2 is a conserved serine/threonine kinase that participates in various cellular processes. Elevated nuclear expression of CK2α has been observed in human carcinomas, but mechanisms for its variable localization in cells are poorly understood. This study demonstrates a functional connection between nuclear CK2 and gene expression in relation to cell proliferation. Growth stimulation of quiescent human fibroblasts identified a pool of CK2 highly phosphorylated at serine 7 of the α subunit that translocated into the nucleus. This phosphorylation appeared essential for its nuclear localization and catalytic activity. Protein signatures associated with nuclear CK2 complexes revealed enrichment of transcription factors and chromatin remodelers that seemed unique during progression through G1 phase of the cell cycle. Chromatin immunoprecipitation-sequencing profiling demonstrated recruitment of CK2α to the active gene locus, more abundantly in late G1 phase than in early G1. Notably, we confirmed the presence of CK2α at transcriptional start sites of core histone genes, growth stimulus-associated genes, and ribosomal RNAs. This study suggests that nuclear CK2α complexes, uniquely constituted during cell cycle progression, are essential for cell proliferation, by activating histone genes, triggering ribosome biogenesis, and then synthesize the components necessary for cell division, specified in the nucleus and also in the nucleolus. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-21_08:00:04.402.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Takeshi Shibata |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine |
| Instrument | TripleTOF 5600; LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-21 10:03:45 | ID requested | |
| ⏵ 1 | 2024-10-21 08:00:04 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CK2, cell cycle, ChIP-Seq, ChIP-qPCR, gene activation, growth-associated genes, histone genes, nano-LC mass spectrometry, nuclear localization, transcription |
Contact List
| Miwako Kato Homma | |
|---|---|
| lab head | |
| Takeshi Shibata | |
| contact affiliation | Sciex |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002361/ |
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