PXD046249-2

PXD046249 is an original dataset announced via ProteomeXchange.

Title	Acoustic Ejection Mass Spectrometry Empowers Ultra-Fast Peptide Biomarker Quantification... A Paradigm shift
Description	There is an urgent need for increased capacity of accurate quantification of proteins to better understand health and disease. Mechanistically, higher throughput is needed to cope with biological variation; clinically the COVID-19 pandemic has underscored the critical demand for high throughput methods for measuring e.g. acute phase response (APR) proteins or the SARS-CoV-2 viral proteins to characterize infection dynamics. Conventional protein biomarker screening approaches generally rely on capture reagents with colorimetric detection strategies (e.g., immunoassays) which are parallelized to allow for rapid throughput. An alternative detection strategy is mass spectrometry coupled with liquid chromatography (LC-MS). However, this is done sequentially, challenging throughput. Acoustic ejection mass spectrometry (AEMS) has emerged as a potential paradigm shift for ultra-fast screening by MS, at throughputs as fast as 1-2 seconds per analyte per sample. Here, we present the first quantitative AEMS assays utilizing peptide immunocapture to enrich 10 APR protein markers from plasma. We quantified 10 proteins from 267 plasma samples in triplicate in 4.8 hours with %CV between 4.2 and 10.5%, representing a 20-fold speed increase over LC-MS. Similarly, we show the capability of direct SARS-CoV-2 peptide quantification in nasopharyngeal swabs. By leveraging the speed of AEMS technology, combined with the enrichment and selectivity provided by peptide immunocapture, this generalizable approach enables both biomarker screening and diagnostic detection on very large cohorts, accelerating our ability to translate biological discoveries into future clinical use.
HostingRepository	PanoramaPublic
AnnounceDate	2024-07-02
AnnouncementXML	Submission_2024-07-02_08:00:03.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bart Van Puyvelde
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Triple Quad 6500+

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-19 19:19:42	ID requested
1	2024-04-15 14:55:35	announced
⏵ 2	2024-07-02 08:00:04	announced	: Added PubMed Id

Van Puyvelde B, Hunter CL, Zhgamadze M, Savant S, Wang YO, Hoedt E, Raedschelders K, Pope M, Huynh CA, Ramanujan VK, Tourtellotte W, Razavi M, Anderson NL, Martens G, Deforce D, Fu Q, Dhaenens M, Van Eyk JE, Acoustic ejection mass spectrometry empowers ultra-fast protein biomarker quantification. Nat Commun, 15(1):5114(2024) [pubmed]

submitter keyword: Mass Spectrometry, SARS-CoV-2, Biomarkers, Acoustic Ejection Mass Spectrometry, Inflammation

Jennifer Van Eyk
contact affiliation	Advanced Clinical Biosystems Research Institute, Smidt Heart Institute, Cedars-Sinai Medical Center
contact email	Jennifer.VanEyk@cshs.org
lab head
Bart Van Puyvelde
contact affiliation	Laboratory of Pharmaceutical Biotechnology, Ghent University
contact email	bart.vanpuyvelde@ugent.be
dataset submitter

Panorama Public dataset URI